AB174291

Anti-EPS15 antibody [EP9280]

RabMAb
What is this?

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(8 Publications)

Rabbit Monoclonal EPS15 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

AF1P, EPS15, Epidermal growth factor receptor substrate 15, Protein Eps15, Protein AF-1p

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP9280

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPS15 also known as Epidermal Growth Factor Receptor Substrate 15 is a critical adaptor protein involved in the process of endocytosis. The protein has a molecular mass of approximately 105 kDa and is highly expressed in various tissues particularly in the nervous system. EPS15 acts as an important player in clathrin-mediated endocytosis by interacting with components of endocytic machinery such as clathrin and AP2 adaptor complexes. It also contains multiple EH domains which facilitate protein-protein interactions needed for the internalization of receptors.

Biological function summary

The EPS15 protein contributes to the regulation of cell surface receptors and is involved in the recycling of these receptors back to the membrane or directing them to lysosomal degradation. This protein partakes in the formation of the clathrin-coated pits which are essential for the invagination of the membrane in the endocytic process. EPS15 works within a complex that aids in trafficking signals within cells allowing cells to maintain homeostasis by dynamically responding to external stimuli.

Pathways

EPS15 significantly influences the endocytosis and EGFR signaling pathways. The protein facilitates the downregulation of receptor tyrosine kinase signaling by mediating the internalization and recycling of the epidermal growth factor receptor (EGFR). EPS15 also interacts with other proteins such as Grb2 and Epsin which are integral to these pathways. The relationship between EPS15 and these pathways highlights its importance in cell communication and proliferation control.

Dysfunction of EPS15 links to cancer and neurological disorders. Abnormalities in EPS15 expression or function can contribute to cancer progression by disrupting EGFR signaling leading to unregulated cell growth and division. In neurological contexts altered EPS15 expression may affect synaptic transmission and faulty receptor trafficking potentially playing a role in neurodegenerative diseases. The interaction between EPS15 and proteins like EGFR and Epsin emphasizes its involvement in pathological conditions suggesting potential therapeutic targets for certain diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.

See full target information EPS15

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:22649 PubMed40595023

2025

RDM1 plays an oncogenic role in clear cell renal cell carcinoma potentially by modulating MCM2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuming Li,Hui Liu,Yujie Wei,Anxin Wang,Chengcheng Lv,Yu Zeng

Advanced biology 8:e2300513 PubMed38494421

2024

The α-1 Adrenergic Receptor Antagonist Doxazosin Attenuates Liver Fibrosis by Alleviating Sinusoidal Capillarization and Liver Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ai-Yuan Xiu,Qian Ding,Chang-Peng Zhu,Chun-Qing Zhang

Neurobiology of pain (Cambridge, Mass.) 12:100109 PubMed36531612

2022

Cell specific regulation of NaV1.7 activity and trafficking in rat nodose ganglia neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Santiago I Loya-López,Paz Duran,Dongzhi Ran,Aida Calderon-Rivera,Kimberly Gomez,Aubin Moutal,Rajesh Khanna

American journal of translational research 14:7324-7335 PubMed36398248

2022

lncRNA EPS15-AS1 affects the biological behavior of liver cancer stem cells by regulating EPS15 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Guodong Liu,Yadong Lan,Xiaoqiang Yin,Tongchui Wu

Molecular brain 14:20 PubMed33478555

2021

Non-SUMOylated CRMP2 decreases Na1.7 currents via the endocytic proteins Numb, Nedd4-2 and Eps15.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberly Gomez,Dongzhi Ran,Cynthia L Madura,Aubin Moutal,Rajesh Khanna

Translational lung cancer research 8:760-774 PubMed32010555

2020

Silence of S1 RNA binding domain 1 represses cell growth and promotes apoptosis in human non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Guowei Cheng,Lei Deng,Yin Yang,Li Sun,Ping Chen,Xiangling He,Dan Su,Nan Bi,Bin Qiu

Autophagy 16:435-450 PubMed31203721

2019

Inactivation of MTOR promotes autophagy-mediated epithelial injury in particulate matter-induced airway inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yin-Fang Wu,Zhou-Yang Li,Ling-Ling Dong,Wei-Jie Li,Yan-Ping Wu,Jing Wang,Hai-Pin Chen,Hui-Wen Liu,Miao Li,Ci-Liang Jin,Hua-Qiong Huang,Song-Min Ying,Wen Li,Hua-Hao Shen,Zhi-Hua Chen

Proceedings of the National Academy of Sciences of 113:E8443-E8452 PubMed27940916

2016

Hierarchical CRMP2 posttranslational modifications control NaV1.7 function.

Applications

Unspecified application

Species

Unspecified reactive species

Erik T Dustrude,Aubin Moutal,Xiaofang Yang,Yuying Wang,May Khanna,Rajesh Khanna

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EPS15 antibody [EP9280]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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Primary Antibodies

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Product promise