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AB284823

Anti-EPS15 antibody [EP9280] - BSA and Azide free

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Rabbit Monoclonal EPS15 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

AF1P, EPS15, Epidermal growth factor receptor substrate 15, Protein Eps15, Protein AF-1p

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPS15 antibody [EP9280] - BSA and Azide free (AB284823)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPS15 antibody [EP9280] - BSA and Azide free (AB284823)

The human prostate cancer section was performed by LeicaDS9800 (Bond™ Polymer Refine Detection). The sections were using Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins, then labelling ab284823 at 1/4000 dilution for 30 mins at room temperature. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP9280

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284823 is the carrier-free version of ab174291

This product has switched from a tissue culture supernatant (hybridoma) to Protein A purified (recombinant) 6th March 2025.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPS15 also known as Epidermal Growth Factor Receptor Substrate 15 is a critical adaptor protein involved in the process of endocytosis. The protein has a molecular mass of approximately 105 kDa and is highly expressed in various tissues particularly in the nervous system. EPS15 acts as an important player in clathrin-mediated endocytosis by interacting with components of endocytic machinery such as clathrin and AP2 adaptor complexes. It also contains multiple EH domains which facilitate protein-protein interactions needed for the internalization of receptors.
Biological function summary

The EPS15 protein contributes to the regulation of cell surface receptors and is involved in the recycling of these receptors back to the membrane or directing them to lysosomal degradation. This protein partakes in the formation of the clathrin-coated pits which are essential for the invagination of the membrane in the endocytic process. EPS15 works within a complex that aids in trafficking signals within cells allowing cells to maintain homeostasis by dynamically responding to external stimuli.

Pathways

EPS15 significantly influences the endocytosis and EGFR signaling pathways. The protein facilitates the downregulation of receptor tyrosine kinase signaling by mediating the internalization and recycling of the epidermal growth factor receptor (EGFR). EPS15 also interacts with other proteins such as Grb2 and Epsin which are integral to these pathways. The relationship between EPS15 and these pathways highlights its importance in cell communication and proliferation control.

Dysfunction of EPS15 links to cancer and neurological disorders. Abnormalities in EPS15 expression or function can contribute to cancer progression by disrupting EGFR signaling leading to unregulated cell growth and division. In neurological contexts altered EPS15 expression may affect synaptic transmission and faulty receptor trafficking potentially playing a role in neurodegenerative diseases. The interaction between EPS15 and proteins like EGFR and Epsin emphasizes its involvement in pathological conditions suggesting potential therapeutic targets for certain diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
See full target information EPS15
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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