Rabbit Polyclonal EPS8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Epidermal growth factor receptor kinase substrate 8 aa 400-600.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Epidermal growth factor receptor kinase substrate 8 aa 400-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q12929
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes.
Alternative names
Epidermal growth factor receptor kinase substrate 8, EPS8
Recommended products
Rabbit Polyclonal EPS8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Epidermal growth factor receptor kinase substrate 8 aa 400-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Epidermal growth factor receptor kinase substrate 8 aa 400-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q12929
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EPS8 also known as Epidermal Growth Factor Receptor Pathway Substrate 8 functions as an adaptor protein that regulates actin cytoskeleton remodeling. This protein has a mass of approximately 97 kDa. EPS8 is expressed in a variety of tissues including those in the brain skin and digestive tract. EPS8 interacts with other proteins at the cellular membrane and in the cytosol playing a role in transmitting signals from receptors on the cell surface to the actin cytoskeleton inside the cell.
- Biological function summary
EPS8 influences cellular behaviors such as migration adhesion and proliferation. It associates with a multiprotein complex that includes other signaling molecules such as SOS1 and ABI1. Through these interactions EPS8 contributes to the regulation of actin filament formation which is important for cell movement and shape. The rigid structure and dynamics of the actin cytoskeleton mainly regulated through EPS8 activity impact various cellular functions.
- Pathways
EPS8 plays a significant role in the epidermal growth factor receptor (EGFR) signaling pathway and the Ras signaling pathway. Within these pathways EPS8 modulates downstream signaling that affects processes like cell growth and survival. It interacts with other proteins such as actin-binding proteins and the SH3 domain-containing proteins which further connect it to diverse signaling cascades.
- Associated diseases and disorders
Disruptions in EPS8 expression or function have associations with cancer progression particularly in colorectal and breast cancers. EPS8 overexpression can lead to increased metastasis and poor prognosis in these cancers. The interaction of EPS8 with proteins like EGFR and other components of signaling pathways highlights its involvement in oncogenic processes. Research into EPS8 offers potential therapeutic strategies in targeting cancers that exhibit aberrant EPS8 activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPS8 antibody (ab244271)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for EPS8 using ab244271 at 1/20 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-EPS8 antibody (ab244271)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for EPS8 (green) using ab244271 at 4 μg/ml in ICC/IF.
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