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AB272642

Anti-EPS8L1 antibody

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Rabbit Polyclonal EPS8L1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EPS8L1 aa 350-500.

View Alternative Names

DRC3, EPS8R1, PP10566, EPS8L1, Epidermal growth factor receptor kinase substrate 8-like protein 1, EPS8-like protein 1, Epidermal growth factor receptor pathway substrate 8-related protein 1, EPS8-related protein 1

2 Images
Western blot - Anti-EPS8L1 antibody (AB272642)
  • WB

Unknown

Western blot - Anti-EPS8L1 antibody (AB272642)

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Western blot - Anti-EPS8L1 antibody (ab272642)

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Western blot - Anti-EPS8L1 antibody (ab272642) at 0.4 µg/mL

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Molecular Weight ladder

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RT4 cells (Human urinary bladder cancer cell line)

Predicted band size: 80 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPS8L1 antibody (AB272642)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPS8L1 antibody (AB272642)

Paraffin-embedded formalin-fixed human placenta tissue stained for EPS8L1 using ab272642 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human EPS8L1 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.

Q8TE68

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPS8L1 also known as Epidermal Growth Factor Receptor Pathway Substrate 8-Like Protein 1 is a protein that weighs approximately 82 kDa. This protein is a member of the EPS8 family known for its capacity to modulate signaling pathways related to cell growth and differentiation. EPS8L1 exhibits expression in various tissues but shows significant expression in epithelial tissues. It plays a mechanical role in linking receptor tyrosine kinases to downstream signaling cascades.
Biological function summary

EPS8L1 contributes to the regulation of actin cytoskeleton dynamics. This protein forms part of a complex with other scaffold proteins impacting cell migration and adhesion processes. Through this role EPS8L1 influences cellular shape and motility which affects tissue organization and repair. Its function in modulating actin structure makes it an important player in maintaining proper cellular architecture.

Pathways

EPS8L1 participates in the EGFR signaling pathway where it acts to mediate the effects of growth factors on cellular mechanics. This pathway includes the interactions of EPS8L1 with proteins such as EGF receptor and SOS1 connecting extracellular signals to intracellular responses that drive proliferation and survival. Additionally EPS8L1 operates within the MAPK/ERK pathway further linking it to cellular growth and differentiation stimuli by interacting with Ras family proteins.

Alterations in EPS8L1 function and expression have associations with cancerous developments particularly involving epithelial-derived tumors. Its interactions with the EGFR and other related receptor proteins contribute to tumor progression and metastasis. Additionally EPS8L1's role in cytoskeletal regulation links it to neurodegenerative diseases where impaired cellular architecture affects neuronal survival and function. These associations highlight the importance of EPS8L1 in maintaining cellular homeostasis and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.
See full target information EPS8L1
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