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AB204952

Anti-Epsin 1 antibody [6F7F9]

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(1 Publication)

Mouse Monoclonal Epsin 1 antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant full length protein, Transfected cell lysate - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Epsin-1 aa 100-300.

View Alternative Names

Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1

3 Images
Flow Cytometry - Anti-Epsin 1 antibody [6F7F9] (AB204952)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Epsin 1 antibody [6F7F9] (AB204952)

Flow cytometric analysis of A431 cells labeling Epsin 1 with ab204952 at 1/200 dilution (green) or negative control (red)

Western blot - Anti-Epsin 1 antibody [6F7F9] (AB204952)
  • WB

Supplier Data

Western blot - Anti-Epsin 1 antibody [6F7F9] (AB204952)

All lanes:

Western blot - Anti-Epsin 1 antibody [6F7F9] (ab204952) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Epsin 1 (106-254) - hIgGFc transfected HEK293 cell lysate

Predicted band size: 60 kDa

false

Western blot - Anti-Epsin 1 antibody [6F7F9] (AB204952)
  • WB

Supplier Data

Western blot - Anti-Epsin 1 antibody [6F7F9] (AB204952)

(Expected MW is 42.4 kDa)

All lanes:

Western blot - Anti-Epsin 1 antibody [6F7F9] (ab204952) at 1/500 dilution

All lanes:

Recombinant Epsin 1 (aa 106-254) protein

Predicted band size: 60 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6F7F9

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human Epsin-1 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6I3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epsin 1 commonly referred to as EPN1 is a protein that primarily participates in the process of endocytosis. It exhibits a molecular mass of approximately 68 kDa. Epsin 1 is found mainly in the cytoplasm but also shows a high expression in the brain indicating its importance in neurological functions. Additionally alternative splicing can lead to different isoforms enriching its functional diversity.
Biological function summary

Epsin 1 works as an endocytic accessory protein that plays a role in clathrin-mediated endocytosis facilitating the internalization of various receptors and membrane proteins. It interacts with clathrin and AP-2 adaptor complex helping to deform the membrane to form vesicles. Epsin 1 is known to bind phospholipids via its ENTH domain which is important for its function at the plasma membrane. This protein does not work alone and is part of larger protein complexes that include other proteins involved in vesicle formation and maturation.

Pathways

Epsin 1 participates in the endocytic pathway essential for the regulation of nutrient uptake and signaling receptor modulation. It coordinates with proteins such as dynamin and adaptin ensuring the proper invagination of the membrane and vesicle scission. Epsin 1 is also linked to the actin cytoskeleton affecting pathways linked to signal transduction and cellular organization.

Epsin 1 has been implicated in cancer and neurological disorders. Abnormal expression levels of Epsin 1 have been associated with tumor progression and metastasis particularly in breast cancer. This correlation suggests that Epsin 1 may influence pathways with epidermal growth factor receptor (EGFR) known for its role in cancer cell proliferation. Moreover its prominent expression in neurons indicates a possible connection to neurodegenerative diseases where similar proteins like amyloid precursor protein (APP) are involved raising interest in its potential role in Alzheimer's disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis (PubMed : 10393179, PubMed : 10557078).
See full target information Epsin-1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:357-367 PubMed39866229

2025

Melanoma cell line-derived exosomal miR-424-5p: a key promoter of angiogenesis through LATS2 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei DU,Qiang Zhang,Jing Zhang,Maierdanjiang Maihemuti,Haiyang He,Renbing Jiang
View all publications
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