AB192908

Anti-Epsin 1 antibody

Name: Anti-Epsin 1 antibody (ab192908) | Abcam
Brand: Abcam Limited
SKU: AB192908

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(1 Publication)

Rabbit Polyclonal Epsin 1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Epsin-1 aa 350 to C-terminus.

View Alternative Names

Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1

2 Images

Supplier Data

Immunoprecipitation - Anti-Epsin 1 antibody (AB192908)

Detection of Epsin 1 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab192908 at 6 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Epsin 1 antibody (ab192908)

Predicted band size: 60 kDa

false

Supplier Data

Western blot - Anti-Epsin 1 antibody (AB192908)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Epsin 1 antibody (ab192908) at 0.1 µg/mL

Lane 1:

human HeLa whole cell lysate at 50 µg

Lane 2:

human 293T whole cell lysate at 50 µg

Lane 3:

human Jurkat whole cell lysate at 50 µg

Lane 4:

mouse TCMK-1 whole cell lysate at 50 µg

Lane 5:

mouse NIH3T3 whole cell lysate at 50 µg

Predicted band size: 60 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human Epsin-1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab192908 was affinity purified using an epitope specific to Epsin 1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epsin 1 commonly referred to as EPN1 is a protein that primarily participates in the process of endocytosis. It exhibits a molecular mass of approximately 68 kDa. Epsin 1 is found mainly in the cytoplasm but also shows a high expression in the brain indicating its importance in neurological functions. Additionally alternative splicing can lead to different isoforms enriching its functional diversity.

Biological function summary

Epsin 1 works as an endocytic accessory protein that plays a role in clathrin-mediated endocytosis facilitating the internalization of various receptors and membrane proteins. It interacts with clathrin and AP-2 adaptor complex helping to deform the membrane to form vesicles. Epsin 1 is known to bind phospholipids via its ENTH domain which is important for its function at the plasma membrane. This protein does not work alone and is part of larger protein complexes that include other proteins involved in vesicle formation and maturation.

Pathways

Epsin 1 participates in the endocytic pathway essential for the regulation of nutrient uptake and signaling receptor modulation. It coordinates with proteins such as dynamin and adaptin ensuring the proper invagination of the membrane and vesicle scission. Epsin 1 is also linked to the actin cytoskeleton affecting pathways linked to signal transduction and cellular organization.

Epsin 1 has been implicated in cancer and neurological disorders. Abnormal expression levels of Epsin 1 have been associated with tumor progression and metastasis particularly in breast cancer. This correlation suggests that Epsin 1 may influence pathways with epidermal growth factor receptor (EGFR) known for its role in cancer cell proliferation. Moreover its prominent expression in neurons indicates a possible connection to neurodegenerative diseases where similar proteins like amyloid precursor protein (APP) are involved raising interest in its potential role in Alzheimer's disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis (PubMed : 10393179, PubMed : 10557078).

See full target information Epsin-1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer research communications 3:2233-2243 PubMed37861290

2023

PRMT5 Inhibitors Regulate DNA Damage Repair Pathways in Cancer Cells and Improve Response to PARP Inhibition and Chemotherapies.

Applications

Unspecified application

Species

Unspecified reactive species

Jack Carter,Michael Hulse,Monisha Sivakumar,Jessica Burtell,Venkat Thodima,Min Wang,Anjana Agarwal,Komali Vykuntam,Jacob Spruance,Neha Bhagwat,Joseph Rager,Bruce Ruggeri,Peggy Scherle,Koichi Ito

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Epsin 1 antibody

Immunoprecipitation - Anti-Epsin 1 antibody (AB192908)

Western blot - Anti-Epsin 1 antibody (AB192908)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise