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AB75879

Anti-Epsin 1 antibody [EPR3023]

4

(1 Review)

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(7 Publications)

Rabbit Recombinant Monoclonal Epsin 1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (AB75879)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (AB75879)

Immunofluorescence staining of HeLa cells with purified ab75879 at a working dilution of 1/50 counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077) used at a dilution of 1/1000. ab7291 a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse 1/1000) shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1 purified ab75879 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2 ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (AB75879)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (AB75879)

Unpurified ab75879 at a 1/100 dilution staining Epsin 1 in SH-SY5Y cells by Immunofluorescence.

Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879)
  • WB

Lab

Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

U-87 MG cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 60 kDa

Observed band size: 94 kDa

false

Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879)
  • WB

Lab

Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution

All lanes:

mouse brain tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 60 kDa

Observed band size: 94 kDa

false

Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879)
  • WB

Lab

Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution

All lanes:

rat brain at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 60 kDa

Observed band size: 94 kDa

false

Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879)
  • WB

Unknown

Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879)

All lanes:

Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/2000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit-HRP at 1/1000 dilution

Predicted band size: 60 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3023

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 100% homology with Epsin 2. Cross-reactivity with Epsin 2 has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p><strong>For unpurified, use 1/100 - 1/250.</strong></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epsin 1 commonly referred to as EPN1 is a protein that primarily participates in the process of endocytosis. It exhibits a molecular mass of approximately 68 kDa. Epsin 1 is found mainly in the cytoplasm but also shows a high expression in the brain indicating its importance in neurological functions. Additionally alternative splicing can lead to different isoforms enriching its functional diversity.
Biological function summary

Epsin 1 works as an endocytic accessory protein that plays a role in clathrin-mediated endocytosis facilitating the internalization of various receptors and membrane proteins. It interacts with clathrin and AP-2 adaptor complex helping to deform the membrane to form vesicles. Epsin 1 is known to bind phospholipids via its ENTH domain which is important for its function at the plasma membrane. This protein does not work alone and is part of larger protein complexes that include other proteins involved in vesicle formation and maturation.

Pathways

Epsin 1 participates in the endocytic pathway essential for the regulation of nutrient uptake and signaling receptor modulation. It coordinates with proteins such as dynamin and adaptin ensuring the proper invagination of the membrane and vesicle scission. Epsin 1 is also linked to the actin cytoskeleton affecting pathways linked to signal transduction and cellular organization.

Epsin 1 has been implicated in cancer and neurological disorders. Abnormal expression levels of Epsin 1 have been associated with tumor progression and metastasis particularly in breast cancer. This correlation suggests that Epsin 1 may influence pathways with epidermal growth factor receptor (EGFR) known for its role in cancer cell proliferation. Moreover its prominent expression in neurons indicates a possible connection to neurodegenerative diseases where similar proteins like amyloid precursor protein (APP) are involved raising interest in its potential role in Alzheimer's disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis (PubMed : 10393179, PubMed : 10557078).
See full target information Epsin-1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113131 PubMed40799387

2025

Cargo-selective regulation of clathrin-mediated endocytosis by AMP-activated protein kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Laura A Orofiamma,Ralph Christian Delos Santos,Ayshin Mehrabi,Nikol Leshchyshyn,Geoffrey G Hesketh,Sadia Rahmani,Aesha Patel,Farnaz Fekri,Rehman Ata,Colin D H Ratcliffe,Mathieu J F Crupi,Lois Mulligan,Morag Park,Anne-Claude Gingras,Costin N Antonescu

Scientific reports 15:23978 PubMed40615537

2025

The role of regulatory B cell/T follicular helper cell balance in thymoma and thymoma-associated myasthenia gravis.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhang,Yuxin Liu,ZiYou Tao,Xinyu Zhang,Yuanguo Wang,Hui Zhang,Jian Li,Zhaoyu Yang,Kai Xiong,Shuning Duan,Zeyang Zhang,Yan Wang,Huan Wang

Proceedings of the National Academy of Sciences of the United States of America 120:e2211368120 PubMed36730202

2023

Clathrin mediates both internalization and vesicular release of triggered T cell receptor at the immunological synapse.

Applications

Unspecified application

Species

Unspecified reactive species

Audun Kvalvaag,Salvatore Valvo,Pablo F Céspedes,David G Saliba,Elke Kurz,Kseniya Korobchevskaya,Michael L Dustin

Proteomics 22:e2200049 PubMed36037246

2022

Proteomic characteristics of plasma and blood cells in natural aging rhesus monkeys.

Applications

Unspecified application

Species

Unspecified reactive species

Na Wu,Fuchuan Xiao,Jing Zhang,Yafei Chi,Yanan Zhai,Baian Chen,Jing Lu

The Journal of cell biology 221: PubMed35238864

2022

Fyn and TOM1L1 are recruited to clathrin-coated pits and regulate Akt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca Cabral-Dias,Stefanie Lucarelli,Karolina Zak,Sadia Rahmani,Gurjeet Judge,John Abousawan,Laura F DiGiovanni,Dafne Vural,Karen E Anderson,Michael G Sugiyama,Gizem Genc,Wanjin Hong,Roberto J Botelho,Gregory D Fairn,Peter K Kim,Costin N Antonescu

Communications biology 3:743 PubMed33293652

2020

Complimentary action of structured and unstructured domains of epsin supports clathrin-mediated endocytosis at high tension.

Applications

Unspecified application

Species

Unspecified reactive species

Jophin G Joseph,Carlos Osorio,Vivian Yee,Ashutosh Agrawal,Allen P Liu

Human molecular genetics 29:274-285 PubMed31816052

2019

A transcriptional and post-transcriptional dysregulation of Dishevelled 1 and 2 underlies the Wnt signaling impairment in type I Gaucher disease experimental models.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Costa,Stefania Bellesso,Susanna Lualdi,Rosa Manzoli,Valeria Pistorio,Mirella Filocamo,Enrico Moro
View all publications
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Product promise

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