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Rabbit Recombinant Monoclonal Epsin 1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 5 publications.


Images

Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879), expandable thumbnail
  • Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879), expandable thumbnail
  • Western blot - Anti-Epsin 1 antibody [EPR3023] (AB75879), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (AB75879), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (AB75879), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Expected
Rat
Tested
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
1/500 - 1/2000
Notes

-

Species
Rat
Dilution info
1/500 - 1/2000
Notes

-

Species
Human
Dilution info
1/500 - 1/2000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

For unpurified, use 1/100 - 1/250.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis (PubMed:10393179, PubMed:10557078).

Alternative names

Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1

Recommended products

Rabbit Recombinant Monoclonal Epsin 1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3023
Purification technique
Affinity purification Protein A
Specificity

The immunogen used for this product shares 100% homology with Epsin 2. Cross-reactivity with Epsin 2 has not been confirmed experimentally.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Epsin 1 commonly referred to as EPN1 is a protein that primarily participates in the process of endocytosis. It exhibits a molecular mass of approximately 68 kDa. Epsin 1 is found mainly in the cytoplasm but also shows a high expression in the brain indicating its importance in neurological functions. Additionally alternative splicing can lead to different isoforms enriching its functional diversity.

Biological function summary

Epsin 1 works as an endocytic accessory protein that plays a role in clathrin-mediated endocytosis facilitating the internalization of various receptors and membrane proteins. It interacts with clathrin and AP-2 adaptor complex helping to deform the membrane to form vesicles. Epsin 1 is known to bind phospholipids via its ENTH domain which is important for its function at the plasma membrane. This protein does not work alone and is part of larger protein complexes that include other proteins involved in vesicle formation and maturation.

Pathways

Epsin 1 participates in the endocytic pathway essential for the regulation of nutrient uptake and signaling receptor modulation. It coordinates with proteins such as dynamin and adaptin ensuring the proper invagination of the membrane and vesicle scission. Epsin 1 is also linked to the actin cytoskeleton affecting pathways linked to signal transduction and cellular organization.

Associated diseases and disorders

Epsin 1 has been implicated in cancer and neurological disorders. Abnormal expression levels of Epsin 1 have been associated with tumor progression and metastasis particularly in breast cancer. This correlation suggests that Epsin 1 may influence pathways with epidermal growth factor receptor (EGFR) known for its role in cancer cell proliferation. Moreover its prominent expression in neurons indicates a possible connection to neurodegenerative diseases where similar proteins like amyloid precursor protein (APP) are involved raising interest in its potential role in Alzheimer's disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879), expandable thumbnail

    Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution

    All lanes: rat brain at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 60 kDa

    Observed band size: 94 kDa

  • Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879), expandable thumbnail

    Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: K562 cell lysate at 10 µg

    Lane 3: U-87 MG cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 60 kDa

    Observed band size: 94 kDa

  • Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879), expandable thumbnail

    Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution

    All lanes: mouse brain tissue lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 60 kDa

    Observed band size: 94 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (ab75879), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (ab75879)

    Immunofluorescence staining of HeLa cells with purified ab75879 at a working dilution of 1/50, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75879 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.

  • Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (ab75879), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (ab75879)

    Unpurified ab75879, at a 1/100 dilution, staining Epsin 1 in SH-SY5Y cells by Immunofluorescence.

  • Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879), expandable thumbnail

    Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)

    All lanes: Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/2000 dilution

    Lane 1: SH-SY5Y cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit-HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 60 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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