Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (AB247415)

This data was developed using ab75879, the same antibody clone in a different buffer formulation.

Immunofluorescence staining of HeLa cells with purified ab75879 at a working dilution of 1/50, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75879 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (AB247415)

This data was developed using ab75879, the same antibody clone in a different buffer formulation.

Unpurified ab75879, at a 1/100 dilution, staining Epsin 1 in SH-SY5Y cells by Immunofluorescence.

• WB

Lab

Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (AB247415)

This data was developed using ab75879, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Epsin 1 antibody [EPR3023] (<a href='/en-us/products/primary-antibodies/epsin-1-antibody-epr3023-ab75879'>ab75879</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

U-87 MG cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 60 kDa

Observed band size: 94 kDa

false

• WB

Lab

Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (AB247415)

This data was developed using ab75879, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Epsin 1 antibody [EPR3023] (<a href='/en-us/products/primary-antibodies/epsin-1-antibody-epr3023-ab75879'>ab75879</a>) at 1/5000 dilution

All lanes:

mouse brain tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 60 kDa

Observed band size: 94 kDa

false

• WB

Lab

Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (AB247415)

This data was developed using ab75879, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Epsin 1 antibody [EPR3023] (<a href='/en-us/products/primary-antibodies/epsin-1-antibody-epr3023-ab75879'>ab75879</a>) at 1/5000 dilution

All lanes:

rat brain at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 60 kDa

Observed band size: 94 kDa

false

• WB

Unknown

Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (AB247415)

This data was developed using ab75879, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Epsin 1 antibody [EPR3023] (<a href='/en-us/products/primary-antibodies/epsin-1-antibody-epr3023-ab75879'>ab75879</a>) at 1/2000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit-HRP at 1/1000 dilution

Predicted band size: 60 kDa

true