AB230546

Anti-Epsin 2 antibody

Name: Anti-Epsin 2 antibody (ab230546) | Abcam
Brand: Abcam Limited
SKU: AB230546
Price: 605 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Epsin 2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EPN2 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA1065, EPN2, Epsin-2, EPS-15-interacting protein 2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epsin 2 antibody (AB230546)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epsin 2 antibody (AB230546)

Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Epsin 2 with ab230546 at a 1 : 100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Epsin 2 antibody (AB230546)

All lanes:

Western blot - Anti-Epsin 2 antibody (ab230546) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Predicted band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human EPN2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O95208

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epsin 2 also known as EPN2 is a protein involved in clathrin-mediated endocytosis where it aids in intracellular trafficking by binding to phospholipids. Weighing approximately 67 kDa Epsin 2 belongs to the epsin family of proteins. This protein is expressed across several tissues with higher levels observed in brain liver and muscle tissues. The presence of a ubiquitin-interacting motif in Epsin 2 allows it to play a specific role in the recruitment of other proteins during cell membrane invagination.

Biological function summary

Epsin 2 participates in regulating membrane curvature and vesicle formation. Epsin 2 is part of a protein complex that includes clathrin AP-2 adaptor complex and other endocytic proteins. By aiding in vesicle formation Epsin 2 contributes to the transport of cargo molecules within the cells. This involvement indicates that Epsin 2 plays an essential role in maintaining cellular homeostasis and responding to environmental changes.

Pathways

Epsin 2 is involved in the endocytic and signaling pathways. It plays a significant role in receptor-mediated endocytosis working with proteins like AP-2 and dynamin. Additionally Epsin 2 interacts with the Notch signaling pathway where it affects receptor internalization. This interaction shows the cross-talk between endocytic processes and signaling pathways essential for cellular communication and development.

Epsin 2 has links to neurodegenerative diseases and cancer. Alterations in Epsin 2 expression have been observed in Alzheimer's disease where disruptions in clathrin-mediated endocytosis contribute to pathogenesis. In cancer Epsin 2 affects cell proliferation and metastasis often interacting with oncogenic proteins like EGFR. These associations illustrate the importance of Epsin 2 in both normal cellular processes and disease pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Plays a role in the formation of clathrin-coated invaginations and endocytosis.

See full target information EPN2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 134: PubMed33526710

2021

Adenine nucleotide translocase regulates airway epithelial metabolism, surface hydration and ciliary function.

Applications

Unspecified application

Species

Unspecified reactive species

Corrine R Kliment,Jennifer M K Nguyen,Mary Jane Kaltreider,YaWen Lu,Steven M Claypool,Josiah E Radder,Frank C Sciurba,Yingze Zhang,Alyssa D Gregory,Pablo A Iglesias,Venkataramana K Sidhaye,Douglas N Robinson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Epsin 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epsin 2 antibody (AB230546)

Western blot - Anti-Epsin 2 antibody (AB230546)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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