Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)

Rabbit Recombinant Monoclonal ER81/ETV1 antibody. Suitable for IHC-P, IHC-Fr, WB and reacts with Transfected cell line - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-ER81/ETV1 antibody [DZR-1-29] (AB322139), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IHC-Fr	WB
Human	Tested	Expected	Tested
Mouse	Tested	Not recommended	Tested
Rat	Tested	Tested	Tested
Transfected cell line - Human	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info -	Notes -

Target data

See full target information ETV1

Function

Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3' (PubMed:7651741). Required for olfactory dopaminergic neuron differentiation; may directly activate expression of tyrosine hydroxylase (TH) (By similarity).

Alternative names

ER81, ETV1, ETS translocation variant 1, Ets-related protein 81

Recommended products

Rabbit Recombinant Monoclonal ER81/ETV1 antibody. Suitable for IHC-P, IHC-Fr, WB and reacts with Transfected cell line - Human, Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

DZR-1-29

Purification technique

Affinity purification Protein A

Specificity

ab322139 shows expression of ETV1 in restricted areas of the cortical brain; potential non-specific staining observed in human tonsil, esophagus and lung.

Unsuitable for mouse IHC-Fr.

Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ER81 also known as ETV1 is a transcription factor with a molecular mass of approximately 54 kDa. This protein is part of the ETS family and characterized by a conserved ETS domain that binds specific DNA sequences. ER81 is expressed highly in neuronal tissues especially in sensory neurons and can also be found in prostate tissue and certain cancer cell lines. Its expression pattern makes it a significant target for research in both developmental biology and oncology.

Biological function summary

ER81 influences many cellular processes by regulating gene expression. It participates in complex transcriptional networks often interacting with other proteins to modulate transcriptional activity. Within various tissues ER81 facilitates neuronal differentiation and plays a role in the development of sensory pathways. It interacts with other transcription factors and co-activators integrating signals from distinct signaling pathways to achieve specific biological outcomes.

Pathways

ER81 influences several critical signaling mechanisms especially in neuronal and cancer-related contexts. It partakes in the MAPK/ERK pathway where it collaborates with other ETS family members like ELK1 to mediate cellular responses to growth factors. ER81 also interfaces with the PI3K/AKT pathway impacting cell survival and proliferation. Through these interactions ER81 helps manage the balance between cell growth and apoptosis.

Associated diseases and disorders

ER81 exhibits connections to certain cancers and neurological diseases. Researchers often study its involvement in prostate cancer due to its elevated expression in tumor tissues. ER81 influences oncogenesis through interactions with proteins like AR (androgen receptor) which modulate tumorigenic pathways. Additionally ER81 has links to neurological disorders due to its role in sensory neuron development although specific proteins involved in these disorders remain under investigation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Western blot - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
All lanes: Western blot - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139) at 1/1000 dilution
Lane 1: C6 (rat glial tumor glial cell) whole lysate at 20 µg
Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole lysate at 20 µg
Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 33-55 kDa
Exposure time: 180s
Immunohistochemistry (Frozen sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (perfused fixed) tissue labeling ER81/ETV1 with ab322139 at 1/50 (9.94 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal image showing no staining on rat spleen. The nuclear counterstain was DAPI (Blue). The section was incubated with ab322139 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Western blot - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: MCF7, T-47D
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18794142).
This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg
Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 33-55 kDa, 36 kDa
Exposure time: 125s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling ER81/ETV1 with ab322139 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining in human cerebellum.

The section was incubated with ab322139 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat colon (perfused fixed) tissue labeling ER81/ETV1 with ab322139 at 1/50 (9.94 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Low expression: confocal image showing positive staining on periperal neurons of rat colon. The nuclear counterstain was DAPI (Blue). The section was incubated with ab322139 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human ETV1 expression vector containing a His-tag. (B) HEK-293T transfected with a human ETV4 expression vector containing a His, Myc-tag. (C) HEK-293T transfected with a human ETV5 expression vector containing a His, Myc-tag. (D) HEK-293T transfected with an empty vector containing a His-tag. tissue labeling ER81/ETV1 with ab322139 at 1/10000 (0.05 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Nuclear staining in (A) HEK-293T transfected with a human ETV1 expression vector containing a His-tag; weak staining in sporadic cells of (B) HEK-293T transfected with a human ETV4 expression vector containing a His, Myc-tag and (C) HEK-293T transfected with a human ETV5 expression vector containing a His, Myc-tag. No staining in (D) HEK-293T transfected with an empty vector containing a His-tag.

The section was incubated with ab322139 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling ER81/ETV1 with ab322139 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: Nearly no staining on rat colon, only showed positive staining in sporadic peripheral neurons.

The section was incubated with ab322139 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (perfused fixed) tissue labeling ER81/ETV1 with ab322139 at 1/50 (9.94 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-ETV1 (ab322139, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732, magenta) on rat cerebellum.

Panel B: anti-ETV1 stained on rat cerebellum.

Panel C: anti-NeuN stained in neurons of rat cerebellum.

Panel D: anti-GFAP stained in astrocytes of rat cerebellum.

The section was incubated in two rounds of staining: in the order of ab322139 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling ER81/ETV1 with ab322139 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse cerebellum.

The section was incubated with ab322139 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ER81/ETV1 with ab322139 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Low expression tissue: Nearly no staining on human colon.

The section was incubated with ab322139 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling ER81/ETV1 with ab322139 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in rat cerebellum.

The section was incubated with ab322139 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of paraffin-embedded (A) U-87 MG (human glioblastoma-astrocytoma epithelial cell). (B) PC-3 (human prostate adenocarcinoma epithelial cell). (C) MCF7 (human breast adenocarcinoma epithelial cell). (D) T-47D (human breast adenocarcinoma epithelial cell). tissue labeling ER81/ETV1 with ab322139 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining in (A) U-87 MG and (B) PC-3; no staining in (C) MCF7 and (D) T-47D.

The section was incubated with ab322139 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling ER81/ETV1 with ab322139 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining in human cerebral cortex.

The section was incubated with ab322139 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [DZR-1-29] (ab322139)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling ER81/ETV1 with ab322139 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: Nearly no staining on mouse colon, only showed positive staining in sporadic peripheral neurons.

The section was incubated with ab322139 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins