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Rabbit Recombinant Monoclonal ER81/ETV1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ER81/ETV1 antibody [EPR28393-132] (AB314874), expandable thumbnail
  • Western blot - Anti-ER81/ETV1 antibody [EPR28393-132] (AB314874), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ER81/ETV1 antibody [EPR28393-132] (AB314874), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (AB314874), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (AB314874), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt (Intra)IHC-Fr
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Tested
Expected
Not recommended
Not recommended
Rat
Tested
Not recommended
Tested
Expected
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3' (PubMed:7651741). Required for olfactory dopaminergic neuron differentiation; may directly activate expression of tyrosine hydroxylase (TH) (By similarity).

Alternative names

ER81, ETV1, ETS translocation variant 1, Ets-related protein 81

Recommended products

Rabbit Recombinant Monoclonal ER81/ETV1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28393-132
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for Human IHC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ER81 also known as ETV1 is a transcription factor with a molecular mass of approximately 54 kDa. This protein is part of the ETS family and characterized by a conserved ETS domain that binds specific DNA sequences. ER81 is expressed highly in neuronal tissues especially in sensory neurons and can also be found in prostate tissue and certain cancer cell lines. Its expression pattern makes it a significant target for research in both developmental biology and oncology.

Biological function summary

ER81 influences many cellular processes by regulating gene expression. It participates in complex transcriptional networks often interacting with other proteins to modulate transcriptional activity. Within various tissues ER81 facilitates neuronal differentiation and plays a role in the development of sensory pathways. It interacts with other transcription factors and co-activators integrating signals from distinct signaling pathways to achieve specific biological outcomes.

Pathways

ER81 influences several critical signaling mechanisms especially in neuronal and cancer-related contexts. It partakes in the MAPK/ERK pathway where it collaborates with other ETS family members like ELK1 to mediate cellular responses to growth factors. ER81 also interfaces with the PI3K/AKT pathway impacting cell survival and proliferation. Through these interactions ER81 helps manage the balance between cell growth and apoptosis.

Associated diseases and disorders

ER81 exhibits connections to certain cancers and neurological diseases. Researchers often study its involvement in prostate cancer due to its elevated expression in tumor tissues. ER81 influences oncogenesis through interactions with proteins like AR (androgen receptor) which modulate tumorigenic pathways. Additionally ER81 has links to neurological disorders due to its role in sensory neuron development although specific proteins involved in these disorders remain under investigation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874), expandable thumbnail

    Western blot - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18794142).

    All lanes: Western blot - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874) at 1/1000 dilution

    Lane 1: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 3: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 55 kDa

    Exposure time: 180s

  • Western blot - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874), expandable thumbnail

    Western blot - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: MCF7
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18794142).
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874) at 1/1000 dilution

    Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 55 kDa

    Exposure time: 92s

  • Immunocytochemistry/ Immunofluorescence - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling ER81/ETV1 with ab314874 at 1/50 (10.34 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
    Confocal image showing nuclear staining in PC-3 cells.
    Negative control: MCF7(PMID:27604655).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ER81/ETV1 with ab314874 at 1/100 (5.17 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Negative control: No staining on rat liver.
    The section was incubated with ab314874 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ER81/ETV1 with ab314874 at 1/100 (5.17 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Negative control: No staining on mouse liver.
    The section was incubated with ab314874 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874)

    Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling ER81/ETV1 with ab314874 at 1/100 (5.17 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on rat cerebellum.
    The section was incubated with ab314874 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874)

    Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling ER81/ETV1 with ab314874 at 1/100 (5.17 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on mouse breast cancer.
    The section was incubated with ab314874 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ER81/ETV1 antibody [EPR28393-132] (ab314874)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling ER81/ETV1 with ab314874 at 1/100 (5.17 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on mouse cerebellum (PMID: 20067496).
    The section was incubated with ab314874 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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