AB69037

Anti-ERAP2 antibody

Name: Anti-ERAP2 antibody (ab69037) | Abcam
Brand: Abcam Limited
SKU: AB69037
Price: 545 USD
Availability: InStock

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(5 Publications)

Mouse Polyclonal ERAP2 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ERAP2.

View Alternative Names

LRAP, ERAP2, Endoplasmic reticulum aminopeptidase 2, Leukocyte-derived arginine aminopeptidase, L-RAP

3 Images

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Western blot - Anti-ERAP2 antibody (AB69037)

All lanes:

Western blot - Anti-ERAP2 antibody (ab69037) at 1/500 dilution

All lanes:

Human kidney lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

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Western blot - Anti-ERAP2 antibody (AB69037)

All lanes:

Western blot - Anti-ERAP2 antibody (ab69037) at 1/500 dilution

Lane 1:

ERAP2 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 110 kDa

Observed band size: 40 kDa

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CiteAb

Western blot - Anti-ERAP2 antibody (AB69037)

ERAP2 western blot using anti-ERAP2 antibody ab69037. Publication image and figure legend from Andrés, A. M., Dennis, M. Y., et al., 2010, PLoS Genet, PubMed 20976248.

ab69037 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab69037 please see the product overview.

Immunoblot analyses of ERAP2 using LCL protein extract.Two LCLs of each ERAP2 genotype (AA, AB, and BB) were tested for protein using primary antibodies specific to : A, ERAP2 (goat polyclonal); B, ERAP2 (mouse polyclonal); and C, ß-actin (see Materials and Methods).

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ERAP2.

Q6P179

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p>Predicted molecular weight: 110 kDa; Isoform 4, 40 kDa.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ERAP2 protein also known as endoplasmic reticulum aminopeptidase 2 plays an important mechanical role in the cell. It belongs to the M1 zinc metallopeptidase family and has a molecular weight of about 110 kDa. ERAP2 is widely expressed in a range of tissues with notable expression in immune-related organs such as the spleen and lymph nodes. It functions to trim peptides in the endoplasmic reticulum preparing them for presentation by major histocompatibility complex (MHC) class I molecules.

Biological function summary

The trimming activity of ERAP2 assists the immune system in recognizing antigens. It works closely with other components of the antigen processing machinery including the proteasome and transporter associated with antigen processing (TAP). ERAP2 can form a functional complex with ERAP1 another aminopeptidase enhancing the efficiency of peptide processing. The interworking of these proteins is vital for maintaining the diversity and specificity of peptides generated for the immune defense.

Pathways

The ERAP2 protein is involved in the antigen processing and presentation pathway a critical component of the body's adaptive immune response. This pathway ensures foreign or aberrant peptides are presented to CD8+ T cells triggering an immune response. Related proteins such as ERAP1 and TAP collaborate with ERAP2 within this pathway highlighting an interconnected mechanism for immune surveillance and activity.

ERAP2 has associations with autoimmune diseases and inflammatory disorders. Variants and dysregulation of ERAP2 expression have links to ankylosing spondylitis a type of arthritis causing inflammation in the spine's joints and ligaments. Similarly altered ERAP2 function is implicated in inflammatory bowel disease. The interplay with ERAP1 is significant here as both proteins contribute to the altered antigen processing observed in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys.

See full target information ERAP2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Arthritis research & therapy 26:36 PubMed38273310

2024

Endoplasmic reticulum aminopeptidase 2 regulates CD4 T cells pyroptosis in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Zhang,Hao Cai,Weiwei Sun,Weijie Wu,Yunyi Nan,Yingchen Ni,Xinyuan Wu,Minhao Chen,Hua Xu,Youhua Wang

Scientific reports 11:9080 PubMed33907276

2021

Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Hsin Liang,Jia-Huei Tsai,Yung-Ming Cheng,Kuang-Yu Chan,Wen-Ling Hsu,Chang-Cheng Lee,Kuo-Hsing Chen,Kun-Huei Yeh

Human molecular genetics 23:6081-7 PubMed24957906

2014

A genome-wide association study identifies a functional ERAP2 haplotype associated with birdshot chorioretinopathy.

Applications

Unspecified application

Species

Human

Jonas J W Kuiper,Jessica Van Setten,Stephan Ripke,Ruben Van 'T Slot,Flip Mulder,Tom Missotten,G Seerp Baarsma,Laurent C Francioli,Sara L Pulit,Carolien G F De Kovel,Ninette Ten Dam-Van Loon,Anneke I Den Hollander,Paulien Huis in het Veld,Carel B Hoyng,Miguel Cordero-Coma,Javier Martín,Victor Llorenç,Bharti Arya,Dhanes Thomas,Steven C Bakker,Roel A Ophoff,Aniki Rothova,Paul I W De Bakker,Tuna Mutis,Bobby P C Koeleman

Nature communications 4:2359 PubMed23965983

2013

p53 increases MHC class I expression by upregulating the endoplasmic reticulum aminopeptidase ERAP1.

Applications

Species

Unspecified reactive species

Bei Wang,Dandan Niu,Liyun Lai,Ee Chee Ren

PLoS genetics 6:e1001157 PubMed20976248

2010

Balancing selection maintains a form of ERAP2 that undergoes nonsense-mediated decay and affects antigen presentation.

Applications

Species

Human

Aida M Andrés,Megan Y Dennis,Warren W Kretzschmar,Jennifer L Cannons,Shih-Queen Lee-Lin,Belen Hurle,Pamela L Schwartzberg,Scott H Williamson,Carlos D Bustamante,Rasmus Nielsen,Andrew G Clark,Eric D Green

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERAP2 antibody

Western blot - Anti-ERAP2 antibody (AB69037)

Western blot - Anti-ERAP2 antibody (AB69037)

Western blot - Anti-ERAP2 antibody (AB69037)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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