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Rabbit Recombinant Monoclonal ERAP2 antibody. Suitable for WB, IHC-P, IP and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAP2 antibody [EPR26475-10] (AB313567), expandable thumbnail
  • Immunoprecipitation - Anti-ERAP2 antibody [EPR26475-10] (AB313567), expandable thumbnail
  • Western blot - Anti-ERAP2 antibody [EPR26475-10] (AB313567), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAP2 antibody [EPR26475-10] (AB313567), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAP2 antibody [EPR26475-10] (AB313567), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIPICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys.

Alternative names

LRAP, ERAP2, Endoplasmic reticulum aminopeptidase 2, Leukocyte-derived arginine aminopeptidase, L-RAP

Recommended products

Rabbit Recombinant Monoclonal ERAP2 antibody. Suitable for WB, IHC-P, IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26475-10
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ERAP2 protein also known as endoplasmic reticulum aminopeptidase 2 plays an important mechanical role in the cell. It belongs to the M1 zinc metallopeptidase family and has a molecular weight of about 110 kDa. ERAP2 is widely expressed in a range of tissues with notable expression in immune-related organs such as the spleen and lymph nodes. It functions to trim peptides in the endoplasmic reticulum preparing them for presentation by major histocompatibility complex (MHC) class I molecules.

Biological function summary

The trimming activity of ERAP2 assists the immune system in recognizing antigens. It works closely with other components of the antigen processing machinery including the proteasome and transporter associated with antigen processing (TAP). ERAP2 can form a functional complex with ERAP1 another aminopeptidase enhancing the efficiency of peptide processing. The interworking of these proteins is vital for maintaining the diversity and specificity of peptides generated for the immune defense.

Pathways

The ERAP2 protein is involved in the antigen processing and presentation pathway a critical component of the body's adaptive immune response. This pathway ensures foreign or aberrant peptides are presented to CD8+ T cells triggering an immune response. Related proteins such as ERAP1 and TAP collaborate with ERAP2 within this pathway highlighting an interconnected mechanism for immune surveillance and activity.

Associated diseases and disorders

ERAP2 has associations with autoimmune diseases and inflammatory disorders. Variants and dysregulation of ERAP2 expression have links to ankylosing spondylitis a type of arthritis causing inflammation in the spine's joints and ligaments. Similarly altered ERAP2 function is implicated in inflammatory bowel disease. The interplay with ERAP1 is significant here as both proteins contribute to the altered antigen processing observed in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAP2 antibody [EPR26475-10] (ab313567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAP2 antibody [EPR26475-10] (ab313567)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERAP2 with ab313567 at 1/100 (5.0 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human kidney. The section was incubated with ab313567 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunoprecipitation - Anti-ERAP2 antibody [EPR26475-10] (ab313567), expandable thumbnail

    Immunoprecipitation - Anti-ERAP2 antibody [EPR26475-10] (ab313567)

    ERAP2 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab313567 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313567 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2: ab313567 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313567 in HepG2 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 50 seconds

    All lanes: Immunoprecipitation - Anti-ERAP2 antibody [EPR26475-10] (ab313567) at 1/30 dilution

    All lanes: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 50s

  • Western blot - Anti-ERAP2 antibody [EPR26475-10] (ab313567), expandable thumbnail

    Western blot - Anti-ERAP2 antibody [EPR26475-10] (ab313567)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: JEG-3 (PMID: 29324974).
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-ERAP2 antibody [EPR26475-10] (ab313567) at 1/1000 dilution

    Lane 1: JAR (human placenta choriocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 5: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 7: JEG-3 (Human placenta epithelial choriocarcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 120 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAP2 antibody [EPR26475-10] (ab313567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAP2 antibody [EPR26475-10] (ab313567)

    Immunohistochemical analysis of paraffin-embedded Panel A HepG2 (hum tissue labeling ERAP2 with ab313567 at 1/500 (1.0 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HepG2 cell pellet, no staining on (B) JEG-3 cell pellet. The section was incubated with ab313567 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAP2 antibody [EPR26475-10] (ab313567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAP2 antibody [EPR26475-10] (ab313567)

    Immunohistochemical analysis of paraffin-embedded Human endometrial ca tissue labeling ERAP2 with ab313567 at 1/100 (5.0 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human endometrial carcinoma. The section was incubated with ab313567 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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