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AB192868

Anti-ERAS antibody [EPR15595]

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(2 Publications)

Rabbit Recombinant Monoclonal ERAS antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

HRAS2, HRASP, ERAS, GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras

2 Images
Western blot - Anti-ERAS antibody [EPR15595] (AB192868)
  • WB

Supplier Data

Western blot - Anti-ERAS antibody [EPR15595] (AB192868)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ERAS antibody [EPR15595] (ab192868) at 1/5000 dilution

All lanes:

F9 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Western blot - Anti-ERAS antibody [EPR15595] (AB192868)
  • WB

Supplier Data

Western blot - Anti-ERAS antibody [EPR15595] (AB192868)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ERAS antibody [EPR15595] (ab192868) at 1/1000 dilution

All lanes:

Recombinant Human ERAS protein (<a href='/en-us/products/unavailable/recombinant-human-eras-protein-ab158695'>ab158695</a>) at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

false

  • Carrier free

    Anti-ERAS antibody [EPR15595] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15595

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERAS also known as Embryonic Stem Cell Ras (EC-Ras) is a member of the Ras protein family. It has a molecular weight of approximately 23 kDa. ERAS is mainly expressed in embryonic stem cells and specific cancer cells. It shares structural similarities with other Ras family proteins having a low GTPase activity that contributes to its role as a molecular switch in signaling pathways. Like other Ras proteins ERAS toggles between active GTP-bound and inactive GDP-bound forms which influences downstream signaling pathways.
Biological function summary

The ERAS protein influences cell proliferation survival and growth in tissues where it is expressed. It is not part of a larger protein complex but rather acts independently to stimulate the phosphoinositide 3-kinase (PI3K) pathway. This activation of the PI3K pathway leads to the downstream activation of Akt a critical effector in cellular growth and survival processes. By promoting these cellular functions ERAS contributes to maintaining the self-renewal properties of stem cells and influencing oncogenic processes in cancer cells.

Pathways

ERAS engages actively with the PI3K/Akt signaling pathway facilitating the survival and growth of cells. It directly interacts with the PI3K protein promoting cellular proliferation. Additionally ERAS has interactions with the MAPK/ERK pathway a well-known pathway linked to cell division and differentiation. The interaction between these pathways involves proteins like Raf and MEK which integrates growth signals coming from activated Ras proteins.

ERAS expression has been associated with certain types of cancers notably germ cell tumors and possibly breast cancer. Through its interaction with the PI3K/Akt signaling pathway ERAS contributes to the oncogenic properties observed in these cancers. Moreover its activities can influence the behavior of proteins such as PTEN and p53 which are well known for their tumor suppressor functions. The modulation of these pathways and interactions ultimately affect cellular processes like apoptosis which can contribute to the development and progression of cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells (By similarity).
See full target information ERAS
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed39146021

2024

PRMT5-mediated FUBP1 methylation accelerates prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Yan,Xun Liu,Xuefeng Qiu,Xuebin Zhang,Jiahui Chen,Kai Xiao,Ping Wu,Chao Peng,Xiaolin Hu,Zengming Wang,Jun Qin,Liming Sun,Luonan Chen,Denglong Wu,Shengsong Huang,Lichen Yin,Zhenfei Li

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34921114

2021

N6-methyladenosine (mA) depletion regulates pluripotency exit by activating signaling pathways in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-Xuan Jin,Rujuan Zuo,Konstantinos Anastassiadis,Arne Klungland,Carsten Marr,Adam Filipczyk
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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