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AB239011

Anti-ERAS antibody [EPR15595] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ERAS antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

HRAS2, HRASP, ERAS, GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras

1 Images
Western blot - Anti-ERAS antibody [EPR15595] - BSA and Azide free (AB239011)
  • WB

Supplier Data

Western blot - Anti-ERAS antibody [EPR15595] - BSA and Azide free (AB239011)

Blocking buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192868).

All lanes:

Western blot - Anti-ERAS antibody [EPR15595] (<a href='/en-us/products/primary-antibodies/eras-antibody-epr15595-ab192868'>ab192868</a>) at 1/5000 dilution

All lanes:

F9 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15595

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab239011 is the carrier-free version of ab192868.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERAS also known as Embryonic Stem Cell Ras (EC-Ras) is a member of the Ras protein family. It has a molecular weight of approximately 23 kDa. ERAS is mainly expressed in embryonic stem cells and specific cancer cells. It shares structural similarities with other Ras family proteins having a low GTPase activity that contributes to its role as a molecular switch in signaling pathways. Like other Ras proteins ERAS toggles between active GTP-bound and inactive GDP-bound forms which influences downstream signaling pathways.
Biological function summary

The ERAS protein influences cell proliferation survival and growth in tissues where it is expressed. It is not part of a larger protein complex but rather acts independently to stimulate the phosphoinositide 3-kinase (PI3K) pathway. This activation of the PI3K pathway leads to the downstream activation of Akt a critical effector in cellular growth and survival processes. By promoting these cellular functions ERAS contributes to maintaining the self-renewal properties of stem cells and influencing oncogenic processes in cancer cells.

Pathways

ERAS engages actively with the PI3K/Akt signaling pathway facilitating the survival and growth of cells. It directly interacts with the PI3K protein promoting cellular proliferation. Additionally ERAS has interactions with the MAPK/ERK pathway a well-known pathway linked to cell division and differentiation. The interaction between these pathways involves proteins like Raf and MEK which integrates growth signals coming from activated Ras proteins.

ERAS expression has been associated with certain types of cancers notably germ cell tumors and possibly breast cancer. Through its interaction with the PI3K/Akt signaling pathway ERAS contributes to the oncogenic properties observed in these cancers. Moreover its activities can influence the behavior of proteins such as PTEN and p53 which are well known for their tumor suppressor functions. The modulation of these pathways and interactions ultimately affect cellular processes like apoptosis which can contribute to the development and progression of cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells (By similarity).
See full target information ERAS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1336282 PubMed38576477

2024

PPARδ agonist protects against osteoarthritis by activating AKT/mTOR signaling pathway-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Guantong Sun,Xiaodong Li,Pengcheng Liu,Yao Wang,Cheng Yang,Shuhong Zhang,Lei Wang,Xiaoqing Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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