AB131490

Anti-ErbB2 / HER2 antibody

Name: Anti-ErbB2 / HER2 antibody (ab131490) | Abcam
Brand: Abcam Limited
SKU: AB131490
Price: 535 USD
Availability: InStock

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(12 Publications)

Rabbit Polyclonal ErbB2 / HER2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human ERBB2 aa 1200 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody (AB131490)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody (AB131490)

Immunohistochemiscal analysis of ErbB2 / HER2 in Human breast cancer tissue labelled with ab131490 at a 1/50 dilution (left image), or preincubated with blocking peptide (right image).

Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody (AB131490)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody (AB131490)

Immunofluorescence analysis of ErbB2 / HER2 in methanol-fixed MCF7 cells labeled using ab131490 at a 1/100 dilution.

Supplier Data

Western blot - Anti-ErbB2 / HER2 antibody (AB131490)

All lanes:

Western blot - Anti-ErbB2 / HER2 antibody (ab131490) at 1/500 dilution

Lane 1:

MCF cell lysate

Lane 2:

SKOV3 cell lysate

Predicted band size: 137 kDa,22 kDa,28 kDa,47 kDa,48 kDa

Observed band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ERBB2 aa 1200 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P04626

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "(methanol fixed)" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab131490 was purified by affinity-chromatography using epitope-specific peptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

See full target information ERBB2

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:16964 PubMed40374836

2025

Modulation of HER2 internalization enhances single-dose antibody-drug potency in HER2 gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Abbey Zidel,Alex Benton,Emma Brown,Shayla Shmuel,Alex Vanover,Sandeep Surendra Panikar,Ron Bose,Haeseong Park,Andrew A Davis,Patrícia M R Pereira

Cancer immunology research 13:729-748 PubMed40249209

2025

Antitumor CD4+ T Helper 1 Cells Target and Control the Outgrowth of Disseminated Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ganesan Ramamoorthi,Marie Catherine Lee,Carly M Farrell,Colin Snyder,Saurabh K Garg,Amy L Aldrich,Vincent Lok,William Dominguez-Viqueira,Sy K Olson-Mcpeek,Marilin Rosa,Namrata Gautam,Shari Pilon-Thomas,Ling Cen,Krithika N Kodumudi,Doris Wiener,Thordur Oskarsson,Ana P Gomes,Robert A Gatenby,Brian J Czerniecki

Molecular pharmaceutics 21:6311-6322 PubMed39471823

2024

[Cu]Cu-NOTA-Trastuzumab and [Zr]Zr-DFO-Trastuzumab in Xenografts with Varied HER2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Simó,Shayla Shmuel,Alex Vanover,Patrícia M R Pereira

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 64:1638-1646 PubMed37385676

2023

Immuno-PET Detects Antibody-Drug Potency on Coadministration with Statins.

Applications

Unspecified application

Species

Unspecified reactive species

Emma L Brown,Shayla Shmuel,Komal Mandleywala,Sandeep Surendra Panikar,Na-Keysha Berry,Yi Rao,Abbey Zidel,Jason S Lewis,Patrícia M R Pereira

Nature communications 13:2526 PubMed35534471

2022

Caveolin-1 temporal modulation enhances antibody drug efficacy in heterogeneous gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Patrícia M R Pereira,Komal Mandleywala,Sébastien Monette,Melissa Lumish,Kathryn M Tully,Sandeep Surendra Panikar,Mike Cornejo,Audrey Mauguen,Ashwin Ragupathi,Nai C Keltee,Marissa Mattar,Yelena Y Janjigian,Jason S Lewis

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 62:366-371 PubMed32646879

2020

Immuno-PET Detects Changes in Multi-RTK Tumor Cell Expression Levels in Response to Targeted Kinase Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia M R Pereira,Jalen Norfleet,Jason S Lewis,Freddy E Escorcia

Molecular pharmaceutics 17:327-337 PubMed31804840

2019

HER2-Targeted PET Imaging and Therapy of Hyaluronan-Masked HER2-Overexpressing Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia M R Pereira,Ashwin Ragupathi,Shayla Shmuel,Komal Mandleywala,Nerissa T Viola,Jason S Lewis

Journal of nuclear medicine : official publication 60:1569-1578 PubMed31171598

2019

Temporal Modulation of HER2 Membrane Availability Increases Pertuzumab Uptake and Pretargeted Molecular Imaging of Gastric Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Patrícia M R Pereira,Komal Mandleywala,Ashwin Ragupathi,Lukas M Carter,Jeroen A C M Goos,Yelena Y Janjigian,Jason S Lewis

Cell death & disease 10:362 PubMed31043588

2019

Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Suli Zhang,Mingming Yue,Yang Li,Jing Bi,Huirong Liu

Oncology reports 41:2717-2728 PubMed30896866

2019

Rab1A promotes proliferation and migration abilities via regulation of the HER2/AKT-independent mTOR/S6K1 pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengwu Cheng,Xinyu Shao,Menglin Xu,Junfeng Wang,Xiaoyi Kuai,Liping Zhang,Jian Wu,Chunli Zhou,Jiading Mao

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ErbB2 / HER2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody (AB131490)

Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody (AB131490)

Western blot - Anti-ErbB2 / HER2 antibody (AB131490)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise