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Knockout Tested Rabbit Recombinant Monoclonal ErbB2 / HER2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (AB251602), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (AB251602), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (AB251602), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (AB251602), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (AB251602), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/13
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Alternative names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal ErbB2 / HER2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
CAL27
Purification technique
Affinity purification Protein A
Concentration
Loading...
Purification notes

Purity is greater than 99%.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251602 is the carrier-free version of Anti-ErbB2 / HER2 antibody [CAL27] ab237715.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

Associated diseases and disorders

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602), expandable thumbnail

    Immunoprecipitation - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602)

    ErbB2 / HER2 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-ErbB2 / HER2 antibody [CAL27] ab237715 at 130 dilution. Western blot was performed from the immunoprecipitate using Anti-ErbB2 / HER2 antibody [CAL27] ab237715 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).
    Lane 2: Anti-ErbB2 / HER2 antibody [CAL27] ab237715 IP in HeLa whole lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ErbB2 / HER2 antibody [CAL27] ab237715 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 15 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ErbB2 / HER2 antibody [CAL27] ab237715).

    All lanes: Immunoprecipitation - Anti-ErbB2 / HER2 antibody [CAL27] (Anti-ErbB2 / HER2 antibody [CAL27] ab237715)

    Predicted band size: 137 kDa

    Observed band size: 180 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602)

    Immunohistochemical analysis of human breast carcinoma tissue labeling ErbB2 / HER2 with Anti-ErbB2 / HER2 antibody [CAL27] ab237715 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the human breast carcinoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with Anti-ErbB2 / HER2 antibody [CAL27] ab237715 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ErbB2 / HER2 antibody [CAL27] ab237715).

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised SK-BR-3 (human mammary gland adenocarcinoma cell line) cells labeling ErbB2 / HER2 with Anti-ErbB2 / HER2 antibody [CAL27] ab237715 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in SK-BR-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ErbB2 / HER2 antibody [CAL27] ab237715).

  • Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602)

    Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methonao-permeabilized SH-BR-3 (human mammary gland adenocarcinoma cell line) cells labeling ErbB2 / HER2 with Anti-ErbB2 / HER2 antibody [CAL27] ab237715 at 1/500 dilution (red) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ErbB2 / HER2 antibody [CAL27] ab237715).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ErbB2 / HER2 using Anti-ErbB2 / HER2 antibody [CAL27] ab237715 at 0.3 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ErbB2 / HER2 antibody [CAL27] ab237715).

  • Western blot - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602)

    False colour image of Western blot: Anti-ErbB2 / HER2 antibody [CAL27] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-ErbB2 / HER2 antibody [CAL27] ab237715 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in ERBB2 knockout cell line. To generate this image, wild-type and ERBB2 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ErbB2 / HER2 antibody [CAL27] ab237715).

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [CAL27] (Anti-ErbB2 / HER2 antibody [CAL27] ab237715) at 1/1000 dilution

    Lane 1: Wild-type HCT 116 cell lysate at 20 µg

    Lane 2: ERBB2 knockout HCT 116 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: SK-BR-3 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 137 kDa

    Observed band size: 180 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ErbB2 / HER2 antibody [CAL27] ab237715).

    Immunohistochemical analysis of mouse breast carcinoma tissue labeling ErbB2 / HER2 with Anti-ErbB2 / HER2 antibody [CAL27] ab237715 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the human breast carcinoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    The section was incubated with Anti-ErbB2 / HER2 antibody [CAL27] ab237715 for 10 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602)

    False colour image of Western blot: Anti-ErbB2 / HER2 antibody [CAL27] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-ErbB2 / HER2 antibody [CAL27] ab237715 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type A549 cell lysates with no signal observed at this size in ERBB2 knockout cell line. To generate this image, wild-type and ERBB2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ErbB2 / HER2 antibody [CAL27] ab237715).

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [CAL27] (Anti-ErbB2 / HER2 antibody [CAL27] ab237715) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: ERBB2 knockout A549 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: SK-BR-3 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 137 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [CAL27] - BSA and Azide free (ab251602)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-ErbB2 / HER2 antibody [CAL27] ab237715).

    Anti-ErbB2 / HER2 antibody [CAL27] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-ErbB2 / HER2 antibody [CAL27] ab237715 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type MCF7 cell lysates with no signal observed at this size in ERBB2 knockout cell line Human ErbB2 / HER2 knockout MCF7 cell line ab286260 (knockout cell lysate ab300208).

    To generate this image, wild-type and ERBB2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [CAL27] (Anti-ErbB2 / HER2 antibody [CAL27] ab237715) at 1/500 dilution

    Lane 1: Wild-type MCF7 cell lysate at 32 µg

    Lane 2: Western blot - Human ErbB2 / HER2 knockout MCF7 cell line (Human ErbB2 / HER2 knockout MCF7 cell line ab286260)

    Lane 2: Western blot - Human ERBB2 knockout MCF7 cell lysate (ab300208)

    Lane 2: ERBB2 knockout MCF7 cell lysate at 32 µg

    Lane 3: SK-BR-3 cell lysate at 16 µg

    Secondary

    All lanes: HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 137 kDa

    Observed band size: 180 kDa

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Product protocols

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