Name: Anti-ErbB2 / HER2 antibody [EP1045Y]
SKU: ab134182
Rating: 4 (4 reviews)

Anti-ErbB2 / HER2 antibody [EP1045Y] is a rabbit recombinant monoclonal antibody that is used to detect ErbB2 / HER2 in ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse samples.

- Specificity confirmed with ERBB2 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 50 publications

Related conjugates and formulations

ab194979
Carrier free
Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free
ab275994
Conjugated
Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y]
ab281782
Conjugated
Alexa Fluor® 555 Anti-ErbB2 / HER2 antibody [EP1045Y]
ab313021
Conjugated
Alexa Fluor® 568 Anti-ErbB2 / HER2 antibody [EP1045Y]
ab281578
Conjugated
Alexa Fluor® 647 Anti-ErbB2 / HER2 antibody [EP1045Y]
ab311741
Conjugated
Alexa Fluor® 594 Anti-ErbB 2 antibody [EP1045Y]
ab321087
Conjugated
Alexa Fluor® 750 Anti-ErbB2 / HER2 antibody [EP1045Y]

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (AB134182), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt	WB	ICC/IF	IHC-P
Human	Tested	Not recommended	Tested	Tested	Tested
Mouse	Expected	Not recommended	Tested	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes For unpurifed use at 1/50. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250 - 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1600	Notes For unpurified use at 1/100 -1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes For unpurified use at 1/100 -1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Target data

See full target information ERBB2

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Alternative names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP1045Y
Purification technique: Affinity purification Protein A
Specificity: ab134182 detects ErbB 2 phosphorylated at Tyr1248 as well as unphosphorylated ErbB 2. Mouse species is recommended based on WB results, we do not guarantee IHC-P for mouse.
Dissociation constant: 3 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse samples.

Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) has been cited over 57 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

The specificity of Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) has been confirmed by testing in knockout samples.

Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) specifically detects ErbB2 / HER2 (UniProt ID: P04626; Molecular weight: 136kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EP1045Y - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free ab194979.

Antibody clone EP1045Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 75 (Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] ab275994, Alexa Fluor® 647 Anti-ErbB2 / HER2 antibody [EP1045Y] ab281578, Alexa Fluor® 555 Anti-ErbB2 / HER2 antibody [EP1045Y] ab281782, Alexa Fluor® 594 Anti-ErbB 2 antibody [EP1045Y] ab311741, ab31321, ab32187).

ErbB2, also known as HER2, is a protein that plays a critical role in the development and progression of certain aggressive cancers, particularly breast cancer. HER2-positive cancers are characterized by the overexpression of this protein, leading to rapid growth and spread of cancer cells.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

Associated diseases and disorders

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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17 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling EErbB2 / HER2 with Purified ab134182 at 1:1600 dilution (0.68 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
ab134182 (purified) at 1:30 dilution (2μg) immunoprecipitating ErbB2 / HER2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab134182 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab134182 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Predicted band size: 137 kDa
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
False colour image of Western blot: Anti-ErbB2 / HER2 antibody [EP1045Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134182 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type HeLa cell lysates with no signal observed at this size in ERBB2 knockout cell line ab255387 (knockout cell lysate ab263758). To generate this image, wild-type and ERBB2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ERBB2 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 137 kDa
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution
All lanes: 4T1(Mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 137 kDa
Observed band size: 180 kDa
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/10000 dilution
All lanes: SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 137 kDa
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution
All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 137 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ErbB2 / HER2 with ab134182 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9.0).
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Immunofluorescent analysis of SKBR cells labelling ErbB2 / HER2 with ab134182 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Immunocytochemistry/Immunofluorescence analysis of SK-BR-3 (human mammary gland adenocarcinoma) labelling ErbB2 / HER2 with purified ab134182 at 1/125. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/10000 dilution
All lanes: SKBR-3 cell lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 137 kDa
OI-RD Scanning - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Western blot: Anti-ErbB2 / HER2 antibody [EP1045Y] staining at 1/500 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134182 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type MCF7 cell lysates with no signal observed at this size in ERBB2 knockout cell line Human ErbB2 / HER2 knockout MCF7 cell line ab286260 (knockout cell lysate ab300208). To generate this image, wild-type and ERBB2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature and washed again four times. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution. This blot was developed with an ultra high-sensitivity ECL substrate kit and imaged with 20 minutes exposure time.
All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/500 dilution
Lane 1: Wild-type MCF7 cell lysate at 32 µg
Lane 2: Western blot - Human ERBB2 knockout MCF7 cell lysate (ab300208)
Lane 2: Western blot - Human ErbB2 / HER2 knockout MCF7 cell line (Human ErbB2 / HER2 knockout MCF7 cell line ab286260)
Lane 2: ERBB2 knockout MCF7 cell lysate at 32 µg
Lane 3: A549 cell lysate at 16 µg
Secondary
All lanes: HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 180 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Negative control image: IHC image of ErbB2 / HER2 staining in a section of formalin-fixed paraffin-embedded MCF7 ErbB2 / HER2 KO cell pellet performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab134182, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
False colour image of Western blot: Anti-ErbB2 / HER2 antibody [EP1045Y] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134182 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type A549 cell lysates with no signal observed at this size in ERBB2 knockout cell line. To generate this image, wild-type and ERBB2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: ERBB2 knockout A549 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: SK-BR-3 cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 180 kDa
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
False colour image of Western blot: Anti-ErbB2 / HER2 antibody [EP1045Y] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134182 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in ERBB2 knockout cell line. To generate this image, wild-type and ERBB2 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution
Lane 1: Wild-type HCT 116 cell lysate at 20 µg
Lane 2: ERBB2 knockout HCT 116 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: SK-BR-3 cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 180 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
IHC image of ErbB2 / HER2 staining in a section of formalin-fixed paraffin-embedded MCF7 cell pellet performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab134182, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
ErbB2 / HER2 western blot using anti-ErbB2 / HER2 antibody [EP1045Y] ab134182. Publication image and figure legend from Wang, H., Lu, J., et al., 2017, BMC Cancer, PubMed 28292264.

ab134182 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab134182 please see the product overview.
AZD4547 and crizotinib synergistically inhibited activation of MAPK/ERK pathway in G03 xenograft derived GC cells. GC cells derived from G03 xenograft were treated with 200nM/L crizotinib or 30nM/L AZD4547, either alone or as a combo treatment(Cri + AZD) for 1 hour. Whole cell lysates were collected and analyzed by western blot. Cell lysates were immunoblotted for phospho- and total protein as indicated