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Knockout Tested Rabbit Recombinant Monoclonal ErbB2 / HER2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications.

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Images

Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (AB194979), expandable thumbnail
  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (AB194979), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (AB194979), expandable thumbnail
  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (AB194979), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (AB194979), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Expected
Expected
Not recommended
Tested
Expected

Tested
Tested

Species

Human

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info
-
Notes

Please check the parent abID, Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182, for more information on dilutions.

Species

Mouse

Dilution info
-
Notes

Please check the parent abID, Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182, for more information on dilutions.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

2 products for Alternative Version

Target data

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Alternative names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal ErbB2 / HER2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EP1045Y

Purification technique

Affinity purification Protein A

Specificity

ab134182 detects ErbB 2 phosphorylated at Tyr1248 as well as unphosphorylated ErbB 2. Mouse species is recommended based on WB results, we do not guarantee IHC-P for mouse.

Dissociation constant
3 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab194979 is the carrier-free version of Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

Associated diseases and disorders

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 (purified) at 1:30 dilution (2μg) immunoprecipitating ErbB2 / HER2 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182).

    All lanes: Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182)

    Predicted band size: 137 kDa

  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    False colour image of Western blot: Anti-ErbB2 / HER2 antibody [EP1045Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type HeLa cell lysates with no signal observed at this size in ERBB2 knockout cell line ab255387 (knockout cell lysate ab263758). To generate this image, wild-type and ERBB2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ERBB2 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 137 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling ErbB2 / HER2 with Purified Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 at 1:1600 dilution (0.68 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182).

  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    All lanes: SKBR-3 (human mammary gland adenocarcinoma) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)

    Predicted band size: 137 kDa

    Observed band size: 185 kDa

    Exposure time: 3s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ErbB2 / HER2 with Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    Immunofluorescent analysis of SKBR cells labelling ErbB2 / HER2 with Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182).

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    Immunocytochemistry/Immunofluorescence analysis of SK-BR-3 (human mammary gland adenocarcinoma) labelling ErbB2 / HER2 with purified Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 at 1/125. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182).

  • OI-RD Scanning - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    OI-RD Scanning - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    False colour image of Western blot: Anti-ErbB2 / HER2 antibody [EP1045Y] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type A549 cell lysates with no signal observed at this size in ERBB2 knockout cell line. To generate this image, wild-type and ERBB2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182).

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: ERBB2 knockout A549 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: SK-BR-3 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 137 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    False colour image of Western blot: Anti-ErbB2 / HER2 antibody [EP1045Y] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in ERBB2 knockout cell line. To generate this image, wild-type and ERBB2 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182).

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182) at 1/1000 dilution

    Lane 1: Wild-type HCT 116 cell lysate at 20 µg

    Lane 2: ERBB2 knockout HCT 116 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: SK-BR-3 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 137 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182).
    Western blot: Anti-ErbB2 / HER2 antibody [EP1045Y] staining at 1/500 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182 was shown to bind specifically to ErbB2 / HER2. A band was observed at 180 kDa in wild-type MCF7 cell lysates with no signal observed at this size in ERBB2 knockout cell line Human ErbB2 / HER2 knockout MCF7 cell line ab286260 (knockout cell lysate ab300208). To generate this image, wild-type and ERBB2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature and washed again four times. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution. This blot was developed with an ultra high-sensitivity ECL substrate kit and imaged with 20 minutes exposure time.

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (Anti-ErbB2 / HER2 antibody [EP1045Y] ab134182) at 1/500 dilution

    Lane 1: Wild-type MCF7 cell lysate at 32 µg

    Lane 2: Western blot - Human ERBB2 knockout MCF7 cell lysate (ab300208)

    Lane 2: Western blot - Human ErbB2 / HER2 knockout MCF7 cell line (Human ErbB2 / HER2 knockout MCF7 cell line ab286260)

    Lane 2: ERBB2 knockout MCF7 cell lysate at 32 µg

    Lane 3: A549 cell lysate at 16 µg

    Secondary

    All lanes: HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 137 kDa

    Observed band size: 180 kDa

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For this product, it's our understanding that no specific protocols are required. You can:

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