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Rabbit Recombinant Monoclonal ErbB2 / HER2 antibody. Suitable for I-ELISA, IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Recombinant full length protein - Human, Human, Mouse, Rat, Recombinant full length protein - Mouse, Recombinant fragment - Human samples. Cited in 13 publications.


Images

Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB214275), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB214275), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB214275), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB214275), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB214275), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAIPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Expected
Expected
Rat
Expected
Expected
Tested
Expected
Expected
Expected
Recombinant fragment - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Mouse
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Mouse, Rat, Recombinant full length protein - Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

1/30

Notes

-

Species

Rat

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Mouse, Recombinant full length protein - Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Mouse, Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250

Notes

-

Expected
Expected

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

1/250

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Mouse, Recombinant full length protein - Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/60

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

1/60

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

1/60

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species

Recombinant full length protein - Mouse, Recombinant full length protein - Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein - Mouse, Recombinant full length protein - Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ErbB2 / HER2 antibody. Suitable for I-ELISA, IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Recombinant full length protein - Human, Human, Mouse, Rat, Recombinant full length protein - Mouse, Recombinant fragment - Human samples. Cited in 13 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19547-12

Purification technique

Affinity purification Protein A

Specificity

Only reacts with weakly in mouse 4T1 cells.
This antibody could recognize ErbB2 and weak cross reaction with ErbB4. 
Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

Associated diseases and disorders

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

  • Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    ErbB 2 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab214275 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab214275 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution

    Lane 1: HeLa whole cell lysate 10μg (Input).

    Lane 2: ab214275 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab214275 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Predicted band size: 137 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ErbB 2 with ab214275 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Membrane staining on Human breast cancer is observed [PMID: 18437174].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)


    Immunofluorescent analysis of 100% methanol-fixed SK-BR-3 (Human mammary gland adenocarcinoma cell line) cells labeling ErbB 2 with ab214275 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing membrane staining on SK-BR-3 cells.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ErbB 2 with ab214275 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Membrane staining on Human breast cancer is observed [PMID: 18437174].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling ErbB 2 with ab214275 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Negative control: No staining on normal Human breast [PMID: 15150568].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed SK-BR-3 (Human mammary gland adenocarcinoma cell line) cells labeling ErbB 2 with ab214275 at 1/60 dilution (red) compared with a RabbitIgG,monoclonal [EPR25A] - Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Blocking and diluting buffer: 5% NFDM/TBST. 

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    All lanes: 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  • Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight is consist with literature PMID :15741050.

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275) at 10 µg

    Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  • Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight is consist with literature PMID :15741050.

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275) at 1/2000 dilution

    All lanes: Human breast cancer lysate at 10 µg

  • Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2: 3 seconds; Lane 3: 10 seconds.

    The molecular weight observed is consistent with the literature PMID :15741050.

    All lanes: Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: Sample: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  • Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Flow cytometry overlay histogram showing T-47D cells stained with ab214275 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab214275) (1x 106 in 100µl at a 1/2500 dilution for 30 min at 22° C.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at a 1/4000 dilution for 30 min at 22° C.

    Isotype control antibody was (black line) Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW blue laser (488nm) and 525/40 bandpass filter.

    This antibody gave a positive signal in T-47D fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

  • Indirect ELISA - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Indirect ELISA - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    ELISA using ab214275 at using 1000 ng/ml an antigen concentration range of 500-0 ng/ml for Human ERBB2 and ERBB4 Recombinant Proteins. Goat Anti-Rabbit IgG (HRL) (1/50000) was used as the secondary antibody. 3,3',5,5'-Tetramethylbenzidine (TMB) used as substrate solution.

  • Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    ErbB2 / HER2 (phospho Y1221 + Y1222) expression can be induced by EGF treatment (PMID: 7728765).

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

    In Western blot, Anti-ErbB2 / HER2 antibody - Total protein control (ab214275) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR28391-67] (Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR28391-67] ab316758) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervix adenocarcinoma epithelial cell) starved for 4 hours whole cell lysate (untreated membrane) at 20 µg

    Lane 2: HeLa starved for 4 hours, then treated with /ml EGF for 15 minutes whole cell lysate (untreated membrane) at 20 µg

    Lane 3: HeLa starved for 4 hours, then treated with /ml EGF for 15 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 180 kDa, 15 kDa

    Exposure time: 81s

  • Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-ErbB2 / HER2 antibody - Total protein control (ab214275) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR28391-67] (Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR28391-67] ab316758) at 1/1000 dilution

    Lane 1: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg

    Lane 2: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 180 kDa, 36 kDa

    Exposure time: 92s

  • Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

    In Western blot, Anti-ErbB2 / HER2 antibody - Total protein control (ab214275) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR28391-67] (Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR28391-67] ab316758) at 1/1000 dilution

    Lane 1: Untreated T47D (human ductal breast epithelial tumor epithelial cell) starved for 4 hours whole cell lysate (untreated membrane) at 20 µg

    Lane 2: T47D starved for 4 hours, then treated with /ml Neuregulin for 10 minutes whole cell lysate (untreated membrane) at 20 µg

    Lane 3: T47D starved for 4 hours, then treated with /ml Neuregulin for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 180 kDa, 15 kDa

    Exposure time: 103s

  • Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    ErbB2 / HER2 (phospho Y1221 + Y1222) expression can be downregulated by lapatinib treatment (PMID: 27738326).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-ErbB2 / HER2 antibody - Total protein control (ab214275) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR28391-67] (Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR28391-67] ab316758) at 1/1000 dilution

    Lane 1: Untreated SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: SK-BR-3 treated with 5uM lapatinib for 24 hours whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 180 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds.

    This antibody could recognize ErbB2 and weak cross reaction with ErbB4.

    Lanes 1 - 2: Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] (ab214275) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-ErbB2 / HER2 antibody [EPR19547-12] - BSA and Azide free (Anti-ErbB2 / HER2 antibody [EPR19547-12] - BSA and Azide free ab222482) at 1/1000 dilution

    Lane 1: His-tagged Recombinant Human ErbB2 protein (aa676-1255)

    Lane 2: His-tagged Recombinant Human ErbB4 protein (aa676-1308)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 137 kDa

    Observed band size: 90 kDa, 80 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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