Mouse Monoclonal ErbB2 / HER2 antibody. Carrier free. Suitable for ICC, Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ERBB2.
Images
![Immunocytochemistry - Anti-ErbB2 / HER2 antibody [ERB2/776] - BSA and Azide free (AB212343), expandable thumbnail](https://content.abcam.com/products/images/erbb2-her2-antibody-erb2-776-bsa-and-azide-free-ab212343--immunocytochemistry-img134159.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-ErbB2 / HER2 antibody [ERB2/776] - BSA and Azide free (AB212343)")
![Flow Cytometry - Anti-ErbB2 / HER2 antibody [ERB2/776] - BSA and Azide free (AB212343), expandable thumbnail](https://content.abcam.com/products/images/erbb2-her2-antibody-erb2-776-bsa-and-azide-free-ab212343--flow-cytometry-img55386.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-ErbB2 / HER2 antibody [ERB2/776] - BSA and Azide free (AB212343)")
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ERBB2. The exact immunogen used to generate this antibody is proprietary information. Database link P04626
Reactivity data
Select an application| ICC | Flow Cyt | |
|---|---|---|
| Human | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
8 products for Alternative Product
Target data
Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Alternative names
HER2, MLN19, NEU, NGL, ERBB2, NGL, NEU, MLN19, HER2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19
Recommended products
Mouse Monoclonal ErbB2 / HER2 antibody. Carrier free. Suitable for ICC, Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ERBB2.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ERBB2. The exact immunogen used to generate this antibody is proprietary information. Database link P04626
- Carrier free
Yes
- Clone number
ERB2/776
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
purified from Bioreactor Concentrate.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.
- Biological function summary
The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.
- Pathways
ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.
- Associated diseases and disorders
ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
![Immunocytochemistry - Anti-ErbB2 / HER2 antibody [ERB2/776] - BSA and Azide free (ab212343), expandable thumbnail](https://content.abcam.com/products/images/erbb2-her2-antibody-erb2-776-bsa-and-azide-free-ab212343--immunocytochemistry-img134159.jpg "Immunocytochemistry - Anti-ErbB2 / HER2 antibody [ERB2/776] - BSA and Azide free (ab212343)")
Immunocytochemistry - Anti-ErbB2 / HER2 antibody [ERB2/776] - BSA and Azide free (ab212343)
Immunofluorescent staining of MeOH-fixed MCF-7 cells labeling ErbB2 / HER2 with ab212343 followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
![Flow Cytometry - Anti-ErbB2 / HER2 antibody [ERB2/776] - BSA and Azide free (ab212343), expandable thumbnail](https://content.abcam.com/products/images/erbb2-her2-antibody-erb2-776-bsa-and-azide-free-ab212343--flow-cytometry-img55386.jpg "Flow Cytometry - Anti-ErbB2 / HER2 antibody [ERB2/776] - BSA and Azide free (ab212343)")
Flow Cytometry - Anti-ErbB2 / HER2 antibody [ERB2/776] - BSA and Azide free (ab212343)
Flow Cytometric analysis of human trypsinized MCF7 (human breast adenocarcinoma cell line) cells labeling ErbB2 / HER2 with ab212343 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
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