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AB264541

Anti-ErbB2 / HER2 antibody [H2Mab-139]

4

(1 Review)

|

(3 Publications)

Mouse Recombinant Monoclonal ErbB2 / HER2 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

3 Images
Flow Cytometry - Anti-ErbB2 / HER2 antibody [H2Mab-139] (AB264541)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-ErbB2 / HER2 antibody [H2Mab-139] (AB264541)

Flow cytometric analysis of HT-29 (human colorectal adenocarcinoma epithelial cell) cells labelling ErbB2 / HER2 with ab264541 at 1/500 dilution (0.1μg) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [H2Mab-139] (AB264541)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [H2Mab-139] (AB264541)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB2 / HER2 with ab264541 at 1/100 dilution followed by a ready to use secondary antibody. No staining on human breast carcinoma without expression of HER2 is observed. The section was incubated with ab264541 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use secondary antibody.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [H2Mab-139] (AB264541)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [H2Mab-139] (AB264541)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB2 / HER2 with ab264541 at 1/100 dilution followed by a ready to use secondary antibody. Membranous staining on human breast carcinoma is observed. The section was incubated with ab264541 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use secondary antibody.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Carrier free

    Anti-ErbB2 / HER2 antibody [H2Mab-139] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

H2Mab-139

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.
Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the ERBB2 gene is a protein tyrosine kinase involved in several cell surface receptor complexes, requiring a coreceptor for ligand binding. It is an essential component of a neuregulin-receptor complex, although neuregulins do not bind to it directly. GP30 is a potential ligand for this receptor. ERBB2 regulates the outgrowth and stabilization of peripheral microtubules (MTs) via the MEMO1-RHOA-DIAPH1 signaling pathway, which, upon activation, phosphorylates and inhibits GSK3B at the cell membrane. This prevents APC and CLASP2 phosphorylation, allowing their association with the cell membrane, facilitating MACF1 localization necessary for microtubule capture and stabilization. In the nucleus, ERBB2 is involved in transcriptional regulation, associating with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter to activate transcription. It is implicated in the transcriptional activation of CDKN1A, involving STAT3 and SRC, and participates in the transcription of rRNA genes by RNA Pol I, thereby enhancing protein synthesis and cell growth. This supplementary information is collated from multiple sources and compiled automatically.
See full target information ERBB2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:336 PubMed40176111

2025

USP15-modified ADMSCs-Exo alleviates chondrocyte damage and effectively relieved osteoarthritis by inducing M2 polarization of macrophages through deubiquitinating FOXC1.

Applications

Unspecified application

Species

Unspecified reactive species

Qibin Liang,Qinghe Ding,Liang Zhao,Jingchao Tan,Wei Niu

mAbs 15:2220466 PubMed37314961

2023

A HER2-targeted antibody-novel DNA topoisomerase I inhibitor conjugate induces durable adaptive antitumor immunity by activating dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoding Tan,Peng Fang,Kaiying Li,Meng You,Yuxia Cao,Hui Xu,Xiaohong Zhu,Lu Wang,Xin Wei,Haiying Wen,Wendi Li,Lei Shi,Xiaowei Sun,Dongan Yu,Huikai Zhu,Zhenzhen Wang,Datao Liu,Hui Shen,Wei Zhou,Maomao An

Monoclonal antibodies in immunodiagnosis and immun 37:59-62 PubMed29315012

2018

Development of an Anti-HER2 Monoclonal Antibody H2Mab-139 Against Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mika K Kaneko,Shinji Yamada,Shunsuke Itai,Yukinari Kato
View all publications
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