Skip to main content

Rabbit Monoclonal ERBB2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Anti-ErbB2 / HER2 (Cytoplasmic domain) antibody [EPR24729-88] - BSA and Azide free (Capture) (AB323579), expandable thumbnail
  • Sandwich ELISA - Anti-ErbB2 / HER2 (Cytoplasmic domain) antibody [EPR24729-88] - BSA and Azide free (Capture) (AB323579), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Rabbit Monoclonal ERBB2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR24729-88

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab323579 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ErbB2 / HER2 (Cytoplasmic domain). The recommended pair for sandwich ELISA is:

Capture: ab323579, Human ErbB2 / HER2 (Cytoplasmic domain) Capture Antibody (unconjugated)

Detector: Anti-ErbB2 / HER2 (Cytoplasmic domain) antibody [EPR24729-57] - BSA and Azide free (Detector) ab323580, Human ErbB2 / HER2 (Cytoplasmic domain) Detector Antibody (unconjugated)

The reference range value is 0.16-20 ng/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Anti-ErbB2 / HER2 (Cytoplasmic domain) antibody [EPR24729-88] - BSA and Azide free (Capture) (ab323579), expandable thumbnail

    Sandwich ELISA - Anti-ErbB2 / HER2 (Cytoplasmic domain) antibody [EPR24729-88] - BSA and Azide free (Capture) (ab323579)

    Interpolated concentration of native ErbB2 / HER2 (Cytoplasmic domain) was measured at different sample concentrations. Undiluted samples are as follows: SK-BR-3 cell extract (50 ?g/mL) and A431 cell extract (1000 ?g/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (Antibody Diluent CP2 ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-ErbB2 / HER2 (Cytoplasmic domain) antibody [EPR24729-88] - BSA and Azide free (Capture) (ab323579), expandable thumbnail

    Sandwich ELISA - Anti-ErbB2 / HER2 (Cytoplasmic domain) antibody [EPR24729-88] - BSA and Azide free (Capture) (ab323579)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com