AB182447

Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y1139 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 1 publication.

View Alternative Names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

4 Images

Lab

Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (AB182447)

This data was developed using ab53290&44; the same antibody clone in a different buffer formulation.

ab53290 at 1/70 dilution immunoprecipitating ErbB2 / HER2 (phospho Y1139) in A431 (human epidermoid carcinoma) whole cell lysate.

Lane 1 (input) : A431 treated with 100 ng/mL EGF for 10 minutes whole cell lysate 10μg

Lane 2 (+) : ab53290 + A431 treated with 100 ng/mL EGF for 10 minutes whole cell lysate

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab53290 in A431 treated with 100 ng/mL EGF for 10 minutes whole cell lysate

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (<a href='/en-us/products/primary-antibodies/erbb2-her2-phospho-y1139-antibody-ep1046y-ab53290'>ab53290</a>)

Predicted band size: 137 kDa

Observed band size: 185 kDa

false

Exposure time: 5s

Lab

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (AB182447)

This data was developed using ab53290, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (<a href='/en-us/products/primary-antibodies/erbb2-her2-phospho-y1139-antibody-ep1046y-ab53290'>ab53290</a>) at 1/500 dilution

Lane 1:

A431 whole cell lysate - untreated at 10 µg

Lane 2:

A431 whole cell lysate - treated with Epidermal Growth Factor (EGF) at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 137 kDa

Observed band size: 185 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (AB182447)

This data was developed using ab53290, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (<a href='/en-us/products/primary-antibodies/erbb2-her2-phospho-y1139-antibody-ep1046y-ab53290'>ab53290</a>) at 1/1000 dilution

Lane 1:

SKBR3 cell lysate at 10 µg

Lane 2:

SKBR3 cell lysate - treated with EGF at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 137 kDa

Observed band size: 185 kDa

false

Unknown

Dot Blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (AB182447)

This data was developed using ab53290, the same antibody clone in a different buffer formulation.Dot blot analysis of ErbB2 / HER2 (pY1139) peptide (Lane 1) and ErbB2 / HER2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y1139) with ab53290 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500. Blocking and dilution buffer : 5% NFDM/TBST. Exposure time : 3 minutes.

Unconjugated

Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1046Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Dot, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

ab182447 is the carrier-free version of ab53290.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the ERBB2 gene is a protein tyrosine kinase involved in several cell surface receptor complexes, requiring a coreceptor for ligand binding. It is an essential component of a neuregulin-receptor complex, although neuregulins do not bind to it directly. GP30 is a potential ligand for this receptor. ERBB2 regulates the outgrowth and stabilization of peripheral microtubules (MTs) via the MEMO1-RHOA-DIAPH1 signaling pathway, which, upon activation, phosphorylates and inhibits GSK3B at the cell membrane. This prevents APC and CLASP2 phosphorylation, allowing their association with the cell membrane, facilitating MACF1 localization necessary for microtubule capture and stabilization. In the nucleus, ERBB2 is involved in transcriptional regulation, associating with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter to activate transcription. It is implicated in the transcriptional activation of CDKN1A, involving STAT3 and SRC, and participates in the transcription of rRNA genes by RNA Pol I, thereby enhancing protein synthesis and cell growth. This supplementary information is collated from multiple sources and compiled automatically.

See full target information ERBB2 phospho Y1139

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of 109:13237-42 PubMed22733765

2012

Functional isolation of activated and unilaterally phosphorylated heterodimers of ERBB2 and ERBB3 as scaffolds in ligand-dependent signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Euisun Park,Kian Kani,Ralf Landgraf

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free

Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (AB182447)

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (AB182447)

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (AB182447)

Dot Blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (AB182447)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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