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AB326662

Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274]

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Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y1139 antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

2 Images
Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (AB326662)
  • WB

Supplier Data

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (AB326662)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : A431 cell lysate
Lane 2 : A431 treated with 100ng/ml EGF 10min
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 138 kDa
Observed band size : 180 kDa
Exposure time : 13 seconds

All lanes:

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (ab326662) at 1/1000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

A431 treated with 100ng/ml EGF 10min at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 138 kDa

Observed band size: 180 kDa

false

Exposure time: 13s

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (AB326662)
  • WB

Supplier Data

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (AB326662)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : ErbB2 (Phospho-Tyr1139) knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (ab326662) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

ErbB2 / HER2 (phospho Y1139) knockout A549 cell lysate at 20 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1274

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
See full target information ERBB2 pY1139
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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