Skip to main content

Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y1221 + Y1222 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 2 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (AB91633), expandable thumbnail
  • Dot Blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (AB91633), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytDotWBIHC-PICC/IF
Human
Not recommended
Not recommended
Expected
Tested
Not recommended
Not recommended
Synthetic peptide
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y1221 + Y1222 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR1047

Purification technique

Affinity purification Protein A

Specificity

ab91633 detects ErbB 2 phosphorylated at tyrosine 1222.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (ab91633), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (ab91633)

    All lanes: Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (ab91633) at 1/5000 dilution

    Lane 1: SKBR3 cell lysate was untreated at 10 µg

    Lane 2: SKBR3 cell lysate was treated with Lambda phosphatase. at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/1000 dilution

    Predicted band size: 137 kDa

  • Dot Blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (ab91633), expandable thumbnail

    Dot Blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (ab91633)

    Primary antibody dilution: 1/1000

    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

    Secondary antibody dilution: 1/2500

    Blocking & dilution buffer: 5% NFDM/TBST

    Lane 1 sample: ErB 2 (pY1221/pY1222) phospho peptide

    Lane 2 sample: ErB 2 non-phospho peptide

    Exposure time: 3 minutes

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com