Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y1221 + Y1222 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.
Images
![Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (AB247528), expandable thumbnail](https://content.abcam.com/products/images/erbb2-her2-phospho-y1221-y1222-antibody-epr1047-bsa-and-azide-free-ab247528--western-blot-img72231.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (AB247528)")
![Dot Blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (AB247528), expandable thumbnail](https://content.abcam.com/products/images/erbb2-her2-phospho-y1221-y1222-antibody-epr1047-bsa-and-azide-free-ab247528--dot-blot-img6072.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (AB247528)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Target data
Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Alternative names
CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19
Recommended products
Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y1221 + Y1222 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1047
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody detects ErbB2 / HER2 phosphorylated at tyrosine 1222.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247528 is the carrier-free version of Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] ab91633.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.
- Biological function summary
The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.
- Pathways
ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.
- Associated diseases and disorders
ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528), expandable thumbnail](https://content.abcam.com/products/images/erbb2-her2-phospho-y1221-y1222-antibody-epr1047-bsa-and-azide-free-ab247528--western-blot-img72231.jpg "Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)")
Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)
This data was developed using Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] ab91633, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] ab91633) at 1/5000 dilution
Lane 1: SKBR3 cell lysate was untreated at 10 µg
Lane 2: SKBR3 cell lysate was treated with Lambda phosphatase. at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 137 kDa
![Dot Blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528), expandable thumbnail](https://content.abcam.com/products/images/erbb2-her2-phospho-y1221-y1222-antibody-epr1047-bsa-and-azide-free-ab247528--dot-blot-img6072.jpg "Dot Blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)")
Dot Blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)
This data was developed using Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] ab91633, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBST Lane 1 sample: ErB 2 (pY1221/pY1222) phospho peptide Lane 2 sample: ErB 2 non-phospho peptide Exposure time: 3 minutes
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