Rabbit Polyclonal ErbB2 / HER2 phospho Y877 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ERBB2 phospho Y877 aa 850-900.
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Publications
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- Computational and structural biotechnology journal 19:2881-28902021Substantial cell apoptosis provoked by naked PAMAM dendrimers in HER2-positive human breast cancer via JNK and ERK1/ERK2 signalling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHadeel Kheraldine,Ishita Gupta,Hashim Alhussain,Aayesha Jabeen,Farhan S Cyprian,Saghir Akhtar,Ala-Eddin Al Moustafa,Ousama RachidPubMed 34093999
- BioMed research international 2019:65954372019Tongxinluo Attenuates Myocardiac Fibrosis after Acute Myocardial Infarction in Rats via Inhibition of Endothelial-to-Mesenchymal Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujie Yin et. al.PubMed 31317035
- Journal of experimental & clinical cancer research 38:2142019GPAA1 promotes gastric cancer progression via upregulation of GPI-anchored protein and enhancement of ERBB signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Xin Zhang et. al.PubMed 31118109
- Cell death & disease 10:3622019Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Sun et. al.PubMed 31043588
- Journal of cellular and molecular medicine 21:1905-19142017Qiliqiangxin protects against anoxic injury in cardiac microvascular endothelial cells via NRG-1/ErbB-PI3K/Akt/mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingfeng Wang et. al.PubMed 28271613
- Oncotarget 7:17301-132016Oncogenic AKT1(E17K) mutation induces mammary hyperplasia but prevents HER2-driven tumorigenesis.Applications:IHCReactive species:Unspecified reactive speciesMaria L Mancini,Evan C Lien,Alex TokerPubMed 27004402
- Molecular pharmacology 87:150-612014Insulin-like growth factor-1 receptor signaling increases the invasive potential of human epidermal growth factor receptor 2-overexpressing breast cancer cells via Src-focal adhesion kinase and forkhead box protein M1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEduardo Sanabria-Figueroa,Siobhan M Donnelly,Kevin C Foy,Meghan C Buss,Robert C Castellino,Elisavet Paplomata,Latonia Taliaferro-Smith,Pravin T P Kaumaya,Rita NahtaPubMed 25391374
- The Journal of biological chemistry 285:7035-442009Her4 and Her2/neu tyrosine kinase domains dimerize and activate in a reconstituted in vitro system.Applications:WBReactive species:Unspecified reactive speciesJohn Monsey,Wei Shen,Paul Schlesinger,Ron BosePubMed 20022944
- Molecular and cellular biology 29:1472-862008The neurofibromatosis 2 protein, merlin, regulates glial cell growth in an ErbB2- and Src-dependent manner.Applications:WBReactive species:MouseS Sean Houshmandi,Ryan J Emnett,Marco Giovannini,David H GutmannPubMed 19103750
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human ERBB2 phospho Y877 aa 850-900. The exact immunogen used to generate this antibody is proprietary information. Database link P04626
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Alternative names
HER2, MLN19, NEU, NGL, ERBB2, NGL, NEU, MLN19, HER2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19
Recommended products
Rabbit Polyclonal ErbB2 / HER2 phospho Y877 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ERBB2 phospho Y877 aa 850-900.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human ERBB2 phospho Y877 aa 850-900. The exact immunogen used to generate this antibody is proprietary information. Database link P04626
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.
- Biological function summary
The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.
- Pathways
ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.
- Associated diseases and disorders
ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)
Staining of phosphorilated ErB 2 on breast carcinoma tissue sections using ab47262 at a 1/50 dilution. Right panel: the antibody was pre-incubated with synthesized phosphopeptide.
Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)
Western blot analysis of HepG2 and HeLa cell lysate using Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262).
All lanes: Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HepG2 cell lysate (blocked with the phospho peptide)
Predicted band size: 137 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)
IHC image of Angiotensin II Type 1 Receptor staining in Mouse Placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47262, 1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)
Western blot analysis of HeLa cell lysate using Anti-ErbB2 (phospho Y877) antibody (ab47262) at 1/500 dilution.
All lanes: Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)
Predicted band size: 137 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262)
Lanes on the left hand side show phosphorylation of ErbB2 at tyrosine 877 (ab47262) in rat Schwann cells upon incubation with 10 ng/ml GGF for 30 minutes (lane 2).
Incubation with 10 ng/ml GGF for 30 minutes does not change the amount of total ErbB2 in rat Schwann cells (as shown on right hand side lanes).
All lanes: Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody (ab47262) at 1/500 dilution
Lane 1: Schwann cell extract untreated at 10 µg
Lane 2: Schwann cell extract treated with 10 ng/ml GGF for 30 minutes. at 10 µg
SecondaryAll lanes: HRP conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 200 kDa
Exposure time: 1min
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