AB108371

Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y]

Name: Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371) | Abcam
Brand: Abcam Limited
SKU: AB108371
Price: 345 USD
Availability: InStock
Rating: 2 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(9 Publications)

Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y877 antibody. Suitable for IHC-P, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 9 publications.

View Alternative Names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

ab108371 at 1/50 dilution staining Human ErbB2 / HER2 (phospho Y877) in SKBR-3 cells by immunofluorescence.

Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB2 / HER2 with purified ab108371 at 1/20. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

ab108371 at 1/250 staining Human ErbB2 / HER2 (phospho Y877) in Human breast ductal infiltrating carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

All lanes:

Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371) at 1/500 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

EGF treated HeLa cell lysate at 10 µg

Lane 3:

SKBR-3 cell lysate at 10 µg

Lane 4:

EGF treated SKBR-3 cell lysate at 10 µg

Predicted band size: 137 kDa

false

Lab

Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab108371) at 1/1000 dilution

Lane 1:

A431 cell lysate - untreated at 10 µg

Lane 2:

A431 cell lysate - treated with Epidermal Growth Factor (EGF) at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/100000 dilution

Predicted band size: 137 kDa

Observed band size: 180 kDa

false

Exposure time: 1min

Lab

Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

Dot blot analysis of ErbB 2 (pY877) peptide (Lane 1) and ErbB2 / HER2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y877) with ab108371 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y]
Carrier free

Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2324Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Dot, IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Perform antigen retrieval before commencing with IHC staining protocol Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% Ethylenediaminetetraacetic acid, disodium, dihydrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the ERBB2 gene is a protein tyrosine kinase involved in several cell surface receptor complexes, requiring a coreceptor for ligand binding. It is an essential component of a neuregulin-receptor complex, although neuregulins do not bind to it directly. GP30 is a potential ligand for this receptor. ERBB2 regulates the outgrowth and stabilization of peripheral microtubules (MTs) via the MEMO1-RHOA-DIAPH1 signaling pathway, which, upon activation, phosphorylates and inhibits GSK3B at the cell membrane. This prevents APC and CLASP2 phosphorylation, allowing their association with the cell membrane, facilitating MACF1 localization necessary for microtubule capture and stabilization. In the nucleus, ERBB2 is involved in transcriptional regulation, associating with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter to activate transcription. It is implicated in the transcriptional activation of CDKN1A, involving STAT3 and SRC, and participates in the transcription of rRNA genes by RNA Pol I, thereby enhancing protein synthesis and cell growth. This supplementary information is collated from multiple sources and compiled automatically.

See full target information ERBB2 phospho Y877

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical medicine 11: PubMed36498537

2022

HER2 Expression in Peritoneal Dissemination of High-Grade Serous Ovarian Carcinoma: A Comparative Study of Immunohistochemical Reactivity Using Four HER2 Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Kyung Yeo,Sup Kim,Heon Jong Yoo,Kwang-Sun Suh,Kyung-Hee Kim

Cell biology international 46:792-805 PubMed35077607

2022

Neuregulin-1 promotes the proliferation, migration, and angiogenesis of human periodontal ligament stem cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Li,Lingling Shang,Wenyan Kang,Lingqian Du,Shaohua Ge

Molecular medicine (Cambridge, Mass.) 27:130 PubMed34666670

2021

Hsp90 up-regulates PD-L1 to promote HPV-positive cervical cancer via HER2/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zeng,Si-Li He,Li-Jie Li,Chen Wang

PloS one 15:e0234991 PubMed32584853

2020

Trastuzumab effects depend on HER2 phosphorylation in HER2-negative breast cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Burguin,Daniela Furrer,Geneviève Ouellette,Simon Jacob,Caroline Diorio,Francine Durocher

BMC cancer 17:535 PubMed28806950

2017

Precision medicine approaches to lung adenocarcinoma with concomitant MET and HER2 amplification.

Applications

Unspecified application

Species

Unspecified reactive species

Doo-Yi Oh,Kyungsoo Jung,Ji-Young Song,Seokhwi Kim,Sang Shin,Yong-Jun Kwon,Ensel Oh,Woong-Yang Park,Sang Yong Song,Yoon-La Choi

Medical science monitor : international medical jo 22:5068-5073 PubMed28008166

2016

Effect of Fu-Zheng-Xiao-Liu Granules on Expression of Human Epidermal Growth Factor Receptor 2 (HER-2) and Proliferation and Apoptosis of Breast Cancer Cell Line SKBR-3.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Mo,Shuangbing Yue,Huan Tian,Hong Lin,Guanglu Zhang,Zili Zhang

The Journal of clinical investigation 126:4444-4459 PubMed27775552

2016

Transcription factor ETV1 is essential for rapid conduction in the heart.

Applications

IHC-Fr

Species

Mouse

Akshay Shekhar,Xianming Lin,Fang-Yu Liu,Jie Zhang,Huan Mo,Lisa Bastarache,Joshua C Denny,Nancy J Cox,Mario Delmar,Dan M Roden,Glenn I Fishman,David S Park

Oncotarget 6:36219-30 PubMed26435481

2015

HER2 as a novel therapeutic target for cervical cancer.

Applications

Species

Unspecified reactive species

Doo-Yi Oh,Seokhwi Kim,Yoon-La Choi,Young Jae Cho,Ensel Oh,Jung-Joo Choi,Kyungsoo Jung,Ji-Young Song,Suzie E Ahn,Byoung-Gie Kim,Duk-Soo Bae,Woong-Yang Park,Jeong-Won Lee,Sangyong Song

Molecular pharmacology 87:150-61 PubMed25391374

2014

Insulin-like growth factor-1 receptor signaling increases the invasive potential of human epidermal growth factor receptor 2-overexpressing breast cancer cells via Src-focal adhesion kinase and forkhead box protein M1.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo Sanabria-Figueroa,Siobhan M Donnelly,Kevin C Foy,Meghan C Buss,Robert C Castellino,Elisavet Paplomata,Latonia Taliaferro-Smith,Pravin T P Kaumaya,Rita Nahta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y]

Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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