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Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y877 antibody. Carrier free. Suitable for ICC/IF, Dot, WB, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples.

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Images

Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (AB247413), expandable thumbnail
  • Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (AB247413), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (AB247413), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (AB247413), expandable thumbnail
  • OI-RD Scanning - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (AB247413), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPDotWBIHC-PFlow Cyt (Intra)
Human
Tested
Not recommended
Expected
Tested
Not recommended
Tested
Synthetic peptide
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Target data

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Alternative names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

Recommended products

Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y877 antibody. Carrier free. Suitable for ICC/IF, Dot, WB, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR2324Y
Purification technique
Affinity purification Protein A
Dissociation constant
8.3 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247413 is the carrier-free version of Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] ab75873.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

Associated diseases and disorders

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413), expandable thumbnail

    Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)

    This data was developed using Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] ab75873, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] (Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] ab75873) at 1/1000 dilution

    Lane 1: HeLa cell lysate, untreated at 10 µg

    Lane 2: HeLa cell lysate, treated with EGF at 10 µg

    Lane 3: SKBR-3 cell lysate, untreated at 10 µg

    Lane 4: SKBR-3 cell lysate, treated with EGF at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 137 kDa

    Observed band size: 180 kDa

  • Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413), expandable thumbnail

    Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)

    This data was developed using Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] ab75873, the same antibody clone in a different buffer formulation.Dot blot analysis of ErbB 2 (pY877) peptide (Lane 1) and ErbB2 / HER2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y877) with Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] ab75873 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.

  • Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)

    This data was developed using Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] ab75873, the same antibody clone in a different buffer formulation.

    Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB 2 with unpurified Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] ab75873 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)

    This data was developed using Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] ab75873, the same antibody clone in a different buffer formulation.Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] ab75873 at 1/50 dilution staining ErbB2 / HER2 in SKBR3 cells by Immunofluorescence.

  • OI-RD Scanning - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413), expandable thumbnail

    OI-RD Scanning - Anti-ErbB2 / HER2 (phospho Y877) antibody [EPR2324Y] - BSA and Azide free (ab247413)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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