AB34641

Anti-ErbB3 / HER3 antibody

Name: Anti-ErbB3 / HER3 antibody (ab34641) | Abcam
Brand: Abcam Limited
SKU: AB34641
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal ErbB3 / HER3 antibody. Suitable for IP, ELISA, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Human ERBB3 aa 1250 to C-terminus.

View Alternative Names

HER3, ERBB3, Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3

1 Images

Supplier Data

Immunoprecipitation - Anti-ErbB3 / HER3 antibody (AB34641)

All lanes:

Immunoprecipitation - Anti-ErbB3 / HER3 antibody (ab34641)

Lane 1:

LNCap prostate cell line no treatment

Lane 2:

LNCap prostate cell line treated with Heregulin beta1 for 15mins at 100ng/ml

Predicted band size: 148 kDa

Observed band size: 180 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, IP, WB

applications

Immunogen

Recombinant Protein within Human ERBB3 aa 1250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P21860

Specificity

The antibody is directed against human HER3 and is useful in determining its presence in immunoprecipitation experiments. This antibody can detect HER3 from human, mouse and rat sources. Reactivity of this antibody with HER3 from other species is unknown.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 5μg. The immunoprecipitation mix contained the antibody, 25 μl of Protein A-agarose beads and 1.0 ml of lysate (lysate contains approximately 1.0 mg of total protein).", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/50000", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Rabbit Anti-HER3 is an IgG fraction antibody purified from monospecific antiserum by Protein A chromatography followed by cross adsorption against GST and extensive dialysis against the buffer.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB3 protein also known as HER3 is a member of the epidermal growth factor receptor (EGFR) family. It belongs to the ErbB receptor tyrosine kinase family but lacks intrinsic kinase activity. The HER3 protein has a molecular weight of approximately 180 kDa. You will find HER3 expressed in various tissues with high expression in the epithelial tissues where it plays a role in normal cellular processes. HER3 interacts with multiple ligands facilitating cellular communication and growth regulation.

Biological function summary

HER3 is essential for activating important signaling pathways that regulate proliferation differentiation and survival. The ErbB3 protein forms heterodimers with other ErbB family members such as ErbB2 (HER2) which is necessary for its signaling capability. The HER3 protein is abundant in heart and nervous tissues indicating its role in cardiovascular and neurological functions. Its activation modulates gene expression through downstream effectors mediated by dimerization.

Pathways

HER3 plays a pivotal role in the PI3K/Akt and MAPK signaling pathways. These pathways are fundamental in cellular responses like growth and survival. HER3 serves as a major partner for HER2 in these signaling cascades enhancing downstream signaling effects. HER3's connection to these pathways makes it a critical node collaborating closely with proteins such as HER2 and EGFR to maintain efficient communication and function within these networks.

HER3 associates strongly with cancer and cardiovascular diseases. Its overexpression in certain cancers such as breast cancer leads to enhanced tumor growth and resistance to therapy. HER3’s relationship with the HER2 protein is especially significant in HER2-positive breast cancer where their interaction drives tumor progression. Additionally alterations in HER3 expression or function impact cardiovascular diseases as it plays a significant role in heart and vascular cell behavior. Recognizing HER3's involvement in these conditions allows for targeted therapeutic approaches and improves disease management strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed : 20682778). May also be activated by CSPG5 (PubMed : 15358134). Involved in the regulation of myeloid cell differentiation (PubMed : 27416908).

See full target information ERBB3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oral surgery, oral medicine, oral pathology and or 129:377-387 PubMed32081558

2020

ErbB3, a possible prognostic factor of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heejin Kim,Joo Yeon Choi,Yoon Chan Rah,Jae-Cheul Ahn,Hyunchul Kim,Woo-Jin Jeong,Soon-Hyun Ahn

Nature communications 6:8303 PubMed26383514

2015

Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.

Applications

Species

Rat

Y Poitelon,S Bogni,V Matafora,G Della-Flora Nunes,E Hurley,M Ghidinelli,B S Katzenellenbogen,C Taveggia,N Silvestri,A Bachi,A Sannino,L Wrabetz,M L Feltri

Molecular cancer 13:220 PubMed25248370

2014

miR-143 and miR-145 synergistically regulate ERBB3 to suppress cell proliferation and invasion in breast cancer.

Applications

Species

Human

Xin Yan,Xi Chen,Hongwei Liang,Ting Deng,Weixu Chen,Suyang Zhang,Minghui Liu,Xiujuan Gao,Yanqing Liu,Chihao Zhao,Xueliang Wang,Nan Wang,Jialu Li,Rui Liu,Ke Zen,Chen-Yu Zhang,Baorui Liu,Yi Ba

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ErbB3 / HER3 antibody

Immunoprecipitation - Anti-ErbB3 / HER3 antibody (AB34641)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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