Skip to main content

Rabbit Polyclonal ErbB3 / HER3 antibody. Suitable for IP, ELISA, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Human ERBB3 aa 1250 to C-terminus.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-ErbB3 / HER3 antibody (AB34641), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Protein within Human ERBB3 aa 1250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P21860

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPELISAWB
Human
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

Use 5μg. The immunoprecipitation mix contained the antibody, 25 μl of Protein A-agarose beads and 1.0 ml of lysate (lysate contains approximately 1.0 mg of total protein).

Expected
Expected

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Expected
Expected

Species

Human

Dilution info

1/500 - 1/2000

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). May also be activated by CSPG5 (PubMed:15358134). Involved in the regulation of myeloid cell differentiation (PubMed:27416908).

Alternative names

Recommended products

Rabbit Polyclonal ErbB3 / HER3 antibody. Suitable for IP, ELISA, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Human ERBB3 aa 1250 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Protein within Human ERBB3 aa 1250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P21860
Purification technique

Affinity purification Protein A

Specificity

The antibody is directed against human HER3 and is useful in determining its presence in immunoprecipitation experiments. This antibody can detect HER3 from human, mouse and rat sources.  Reactivity of this antibody with HER3 from other species is unknown.

Concentration
Loading...
Purification notes

Rabbit Anti-HER3 is an IgG fraction antibody purified from monospecific antiserum by Protein A chromatography followed by cross adsorption against GST and extensive dialysis against the buffer.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ErbB3 protein also known as HER3 is a member of the epidermal growth factor receptor (EGFR) family. It belongs to the ErbB receptor tyrosine kinase family but lacks intrinsic kinase activity. The HER3 protein has a molecular weight of approximately 180 kDa. You will find HER3 expressed in various tissues with high expression in the epithelial tissues where it plays a role in normal cellular processes. HER3 interacts with multiple ligands facilitating cellular communication and growth regulation.

Biological function summary

HER3 is essential for activating important signaling pathways that regulate proliferation differentiation and survival. The ErbB3 protein forms heterodimers with other ErbB family members such as ErbB2 (HER2) which is necessary for its signaling capability. The HER3 protein is abundant in heart and nervous tissues indicating its role in cardiovascular and neurological functions. Its activation modulates gene expression through downstream effectors mediated by dimerization.

Pathways

HER3 plays a pivotal role in the PI3K/Akt and MAPK signaling pathways. These pathways are fundamental in cellular responses like growth and survival. HER3 serves as a major partner for HER2 in these signaling cascades enhancing downstream signaling effects. HER3's connection to these pathways makes it a critical node collaborating closely with proteins such as HER2 and EGFR to maintain efficient communication and function within these networks.

Associated diseases and disorders

HER3 associates strongly with cancer and cardiovascular diseases. Its overexpression in certain cancers such as breast cancer leads to enhanced tumor growth and resistance to therapy. HER3’s relationship with the HER2 protein is especially significant in HER2-positive breast cancer where their interaction drives tumor progression. Additionally alterations in HER3 expression or function impact cardiovascular diseases as it plays a significant role in heart and vascular cell behavior. Recognizing HER3's involvement in these conditions allows for targeted therapeutic approaches and improves disease management strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunoprecipitation - Anti-ErbB3 / HER3 antibody (ab34641), expandable thumbnail

    Immunoprecipitation - Anti-ErbB3 / HER3 antibody (ab34641)

    All lanes: Immunoprecipitation - Anti-ErbB3 / HER3 antibody (ab34641)

    Lane 1: LNCap prostate cell line no treatment

    Lane 2: LNCap prostate cell line treated with Heregulin beta1 for 15mins at 100ng/ml

    Developed using the ECL technique.

    Predicted band size: 148 kDa

    Observed band size: 180 kDa

    Exposure time: 1min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com