AB32121

Anti-ErbB3 / HER3 antibody [E186]

Name: Anti-ErbB3 / HER3 antibody [E186] (ab32121) | Abcam
Brand: Abcam Limited
SKU: AB32121
Price: 365 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(2 Reviews)

(26 Publications)

Rabbit Recombinant Monoclonal ErbB3 / HER3 antibody. Suitable for WB and reacts with Human, Rat samples. Cited in 26 publications.

View Alternative Names

HER3, ERBB3, Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3

4 Images

Lab

Western blot - Anti-ErbB3 / HER3 antibody [E186] (AB32121)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : ERBB3 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : MCF7 whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab32121 observed at 185 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab32121 was shown to specifically recognize ERBB3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when knock out samples were examined. Wild-type and ERBB3 knockout samples were subjected to SDS-PAGE. ab32121 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ErbB3 / HER3 antibody [E186] (ab32121)

Predicted band size: 148 kDa

false

Lab

Western blot - Anti-ErbB3 / HER3 antibody [E186] (AB32121)

All lanes:

Western blot - Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 2.685 µg/mL

Lane 1:

MDA-MB-435S (human mammary gland ductal carcinoma melanocyte) whole cell lysate at 20 µg

Lane 2:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 148 kDa

false

Exposure time: 37s

Unknown

Western blot - Anti-ErbB3 / HER3 antibody [E186] (AB32121)

All lanes:

Western blot - Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/500 dilution

All lanes:

MCF-7 cell lysate

Predicted band size: 148 kDa

Observed band size: 185 kDa

false

Lab

Western blot - Anti-ErbB3 / HER3 antibody [E186] (AB32121)

All lanes:

Western blot - Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/1000 dilution

All lanes:

MDA-MB-435S (human ductal carcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 148 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E186

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab32121 recognises ErbB3/HER3. WB samples: should avoid boiling

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB3 protein also known as HER3 is a member of the epidermal growth factor receptor (EGFR) family. It belongs to the ErbB receptor tyrosine kinase family but lacks intrinsic kinase activity. The HER3 protein has a molecular weight of approximately 180 kDa. You will find HER3 expressed in various tissues with high expression in the epithelial tissues where it plays a role in normal cellular processes. HER3 interacts with multiple ligands facilitating cellular communication and growth regulation.

Biological function summary

HER3 is essential for activating important signaling pathways that regulate proliferation differentiation and survival. The ErbB3 protein forms heterodimers with other ErbB family members such as ErbB2 (HER2) which is necessary for its signaling capability. The HER3 protein is abundant in heart and nervous tissues indicating its role in cardiovascular and neurological functions. Its activation modulates gene expression through downstream effectors mediated by dimerization.

Pathways

HER3 plays a pivotal role in the PI3K/Akt and MAPK signaling pathways. These pathways are fundamental in cellular responses like growth and survival. HER3 serves as a major partner for HER2 in these signaling cascades enhancing downstream signaling effects. HER3's connection to these pathways makes it a critical node collaborating closely with proteins such as HER2 and EGFR to maintain efficient communication and function within these networks.

HER3 associates strongly with cancer and cardiovascular diseases. Its overexpression in certain cancers such as breast cancer leads to enhanced tumor growth and resistance to therapy. HER3’s relationship with the HER2 protein is especially significant in HER2-positive breast cancer where their interaction drives tumor progression. Additionally alterations in HER3 expression or function impact cardiovascular diseases as it plays a significant role in heart and vascular cell behavior. Recognizing HER3's involvement in these conditions allows for targeted therapeutic approaches and improves disease management strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed : 20682778). May also be activated by CSPG5 (PubMed : 15358134). Involved in the regulation of myeloid cell differentiation (PubMed : 27416908).

See full target information ERBB3

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:16964 PubMed40374836

2025

Modulation of HER2 internalization enhances single-dose antibody-drug potency in HER2 gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Abbey Zidel,Alex Benton,Emma Brown,Shayla Shmuel,Alex Vanover,Sandeep Surendra Panikar,Ron Bose,Haeseong Park,Andrew A Davis,Patrícia M R Pereira

Signal transduction and targeted therapy 9:32 PubMed38351062

2024

Docking protein 6 (DOK6) selectively docks the neurotrophic signaling transduction to restrain peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Guo,Pan Xiang,Xiaojiao Sun,Wei Liu,Jiafeng Zhou,Bin Yin,Lin Hou,Boqin Qiang,Huiliang Li,Pengcheng Shu,Xiaozhong Peng

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 64:1638-1646 PubMed37385676

2023

Immuno-PET Detects Antibody-Drug Potency on Coadministration with Statins.

Applications

Unspecified application

Species

Unspecified reactive species

Emma L Brown,Shayla Shmuel,Komal Mandleywala,Sandeep Surendra Panikar,Na-Keysha Berry,Yi Rao,Abbey Zidel,Jason S Lewis,Patrícia M R Pereira

Acta biochimica Polonica 70:261-269 PubMed37331014

2023

β-catenin promotes resistance to trastuzumab in breast cancer cells through enhancing interaction between HER2 and SRC.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Hao,Jialu Zheng,Xiaoqin Yu,Zhixin Li,Guanghui Ren

Nature communications 13:2526 PubMed35534471

2022

Caveolin-1 temporal modulation enhances antibody drug efficacy in heterogeneous gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Patrícia M R Pereira,Komal Mandleywala,Sébastien Monette,Melissa Lumish,Kathryn M Tully,Sandeep Surendra Panikar,Mike Cornejo,Audrey Mauguen,Ashwin Ragupathi,Nai C Keltee,Marissa Mattar,Yelena Y Janjigian,Jason S Lewis

International journal of medical sciences 19:525-536 PubMed35370472

2022

RIP1-dependent Apoptosis and Differentiation Regulated by Skp2 and Akt/GSK3β in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Wenran Dan,Liang Zhong,Zhonghui Zhang,Peng Wan,Yang Lu,Xiao Wang,Zhenyan Liu,Xuan Chu,Beizhong Liu

Journal of clinical laboratory analysis 35:e23952 PubMed34403532

2021

AC016405.3 functions as an oncogenic long non-coding RNA by regulating ERBB3 via sponging miR-22-3p in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wei,Jie Wang,Qi He,Lei Liu,Zhiwei Wang

Aging 13:14219-14233 PubMed33988127

2021

LncRNA AK020546 protects against cardiac ischemia-reperfusion injury by sponging miR-350-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Meiqi Zhang,Kang Cheng,Huan Chen,Jianfeng Tu,Ye Shen,Lingxiao Pang,Weihua Wu,Zhenfei Yu

Cell death & disease 11:1008 PubMed33230102

2020

Exosomal circ_DLGAP4 promotes diabetic kidney disease progression by sponging miR-143 and targeting ERBB3/NF-κB/MMP-2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shoujun Bai,Xiaoyan Xiong,Bo Tang,Tingting Ji,Xiaoying Li,Xiaolei Qu,Weiliang Li

Folia neuropathologica 58:176-193 PubMed32729296

2020

Tanshinone IIA alleviates the damage of neurocytes by targeting GLUT1 in ischaemia reperfusion model (in vivo and in vitro experiments).

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Haibo Tong,Xiangyang Wang,Xinxing Wang,Yang Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ErbB3 / HER3 antibody [E186]

Western blot - Anti-ErbB3 / HER3 antibody [E186] (AB32121)

Western blot - Anti-ErbB3 / HER3 antibody [E186] (AB32121)

Western blot - Anti-ErbB3 / HER3 antibody [E186] (AB32121)

Western blot - Anti-ErbB3 / HER3 antibody [E186] (AB32121)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (26)

Product promise