Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free

• WB

Lab

Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (AB189333)

This data was developed using ab32121, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)

Lane 2 : ERBB3 knockout HAP1 whole cell lysate (20 μg)

Lane 3 : MCF7 whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab32121 observed at 185 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab32121 was shown to specifically recognize ERBB3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when knock out samples were examined. Wild-type and ERBB3 knockout samples were subjected to SDS-PAGE. ab32121 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ErbB3 / HER3 antibody [E186] (<a href='/en-us/products/primary-antibodies/erbb3-her3-antibody-e186-ab32121'>ab32121</a>)

Predicted band size: 148 kDa

false

• WB

Lab

Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (AB189333)

This data was developed using ab32121, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ErbB3 / HER3 antibody [E186] (<a href='/en-us/products/primary-antibodies/erbb3-her3-antibody-e186-ab32121'>ab32121</a>) at 2.685 µg/mL

Lane 1:

MDA-MB-435S (human mammary gland ductal carcinoma melanocyte) whole cell lysate at 20 µg

Lane 2:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 148 kDa

false

Exposure time: 37s

• WB

Unknown

Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (AB189333)

This data was developed using ab32121, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ErbB3 / HER3 antibody [E186] (<a href='/en-us/products/primary-antibodies/erbb3-her3-antibody-e186-ab32121'>ab32121</a>) at 1/500 dilution

All lanes:

MCF-7 cell lysate

Predicted band size: 148 kDa

Observed band size: 185 kDa

false

• WB

Lab

Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (AB189333)

This data was developed using ab32121, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ErbB3 / HER3 antibody [E186] (<a href='/en-us/products/primary-antibodies/erbb3-her3-antibody-e186-ab32121'>ab32121</a>) at 1/1000 dilution

All lanes:

MDA-MB-435S (human ductal carcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 148 kDa

false