Anti-ErbB3 / HER3 antibody [SP71]

6 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [SP71] (ab93739)

  Membranous staining on paraffin-embedded sections of human small intestine. The section was incubated with ab93739 at 1:100 dilution (0.7 μg/ml) for 30 mins at room temperature. The secondary antibody staining was achieved with Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Hematoxylin was used as a counter stain.
  

  Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [SP71] (ab93739)

  An immunohistochemical staining of human small intestine labelling ErbB3 / HER3 (SP71) with ab93739 at dilution 1:100.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [SP71] (ab93739)

  ab93739 at 1/100 dilution staining ErbB 3 in formalin-fixed, paraffin-embedded Human small intestine tissue.

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  Flow Cytometry (Intracellular) - Anti-ErbB3 / HER3 antibody [SP71] (ab93739)

  Flow cytometry analysis of MCF7 (human breast adenocarcinoma epithelial cell) labeling ErbB3 / HER3 with purified ab93739 at 1/50 dilution (10.5 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlableled control -Unlabelled cells / blue.

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  Multiplex immunohistochemistry - Anti-ErbB3 / HER3 antibody [SP71] (ab93739)

  Fluorescence multiplex immunohistochemical analysis of the human jejunum (Formalin/PFA-fixed paraffin-embedded sections).
  
  Panel A: merged staining of anti-Muc2 (Anti-MUC2 antibody [EPR23479-47] ab272692, magenta; Opal™690), anti-Eph receptor B2 (Anti-Eph receptor B2 antibody [EPR22427-268] ab252935, green; Opal™520) and anti-ErbB3 / HER3 (Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free ab236220, red; Opal™570) on human jejunum. Panel B: anti-Muc2 stained on goblet cells. Panel C: anti-Eph receptor B2 stained on intestinal stem and progenitor cells. Panel D: anti-ErbB3 / HER3 stained on epithelial cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
  
  The section was incubated in three rounds of staining: in the order of Anti-MUC2 antibody [EPR23479-47] ab272692 at 1/2000 dilution (0.252 μg/ml), Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1/1000 dilution  (0.527 μg/ml), and Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free ab236220 at 1/3000 dilution (0.75 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
  
  This data was developed using Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free ab236220, the same antibody clone in a different buffer formulation.

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  Multiplex immunohistochemistry - Anti-ErbB3 / HER3 antibody [SP71] (ab93739)

  Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections).
  
  Panel A: merged staining of anti-Muc2 (Anti-MUC2 antibody [EPR23479-47] ab272692, magenta; Opal™690), anti-Eph receptor B2 (Anti-Eph receptor B2 antibody [EPR22427-268] ab252935, green; Opal™520) and anti-ErbB3 / HER3 (Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free ab236220, red; Opal™570) on human colon. Panel B: anti-Muc2 stained on goblet cells. Panel C: anti-Eph receptor B2 stained on intestinal stem and progenitor cells. Panel D: anti-ErbB3 / HER3 stained on epithelial cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
  
  The section was incubated in three rounds of staining: in the order of Anti-MUC2 antibody [EPR23479-47] ab272692 at 1/2000 dilution (0.252 μg/ml), Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1/1000 dilution  (0.527 μg/ml), and Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free ab236220 at 1/3000 dilution (0.75 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
  
  This data was developed using Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free ab236220, the same antibody clone in a different buffer formulation.