Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free (AB236220)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for ErbB3 / HER3 using ab93739 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab93739).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free (AB236220)

This data was developed using ab93739, the same antibody clone in a different buffer formulation.

Membranous staining on paraffin-embedded sections of human small intestine. The section was incubated with ab93739 at 1 : 100 dilution (0.7 μg/ml) for 30 mins at room temperature. The secondary antibody staining was achieved with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free (AB236220)

Intracellular Flow Cytometry analysis of MCF7 (human breast adenocarcinoma epithelial cell) labeling ErbB3 / HER3 with purified ab93739 at 1/50 dilution (10.5μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab93739).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free (AB236220)

ab93739 at 1/100 dilution staining ErbB3 / HER3 in formalin-fixed, paraffin-embedded Human small intestine tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab93739).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free (AB236220)

Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Muc2 (ab272692, magenta; Opal™690), anti-Eph receptor B2 (ab252935, green; Opal™520) and anti-ErbB3 / HER3 (ab236220, red; Opal™570) on human colon. Panel B : anti-Muc2 stained on goblet cells. Panel C : anti-Eph receptor B2 stained on intestinal stem and progenitor cells. Panel D : anti-ErbB3 / HER3 stained on epithelial cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab272692 at 1/2000 dilution (0.252 μg/ml), ab252935 at 1/1000 dilution  (0.527 μg/ml), and ab236220 at 1/3000 dilution (0.75 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free (AB236220)

Fluorescence multiplex immunohistochemical analysis of the human jejunum (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Muc2 (ab272692, magenta; Opal™690), anti-Eph receptor B2 (ab252935, green; Opal™520) and anti-ErbB3 / HER3 (ab236220, red; Opal™570) on human jejunum. Panel B : anti-Muc2 stained on goblet cells. Panel C : anti-Eph receptor B2 stained on intestinal stem and progenitor cells. Panel D : anti-ErbB3 / HER3 stained on epithelial cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab272692 at 1/2000 dilution (0.252 μg/ml), ab252935 at 1/1000 dilution  (0.527 μg/ml), and ab236220 at 1/3000 dilution (0.75 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.