AB76469

Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325]

RabMAb
Recombinant
What is this?

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(10 Publications)

Rabbit Recombinant Monoclonal ErbB3 / HER3 phospho Y1289 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 10 publications.

View Alternative Names

HER3, ERBB3, Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (AB76469)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (AB76469)

Immunofluorescent staining of MCF7 cells using ab76469 at 1/100 dilution.

Unknown

Western blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (AB76469)

All lanes:

Western blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469) at 1/5000 dilution

Lane 1:

SKBR3 cell lysate untreated at 10 µg

Lane 2:

SKBR3 cell lysate treated with Neuregulin at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 148 kDa

Observed band size: 185 kDa

false

Carrier free

Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2325

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects ErbB 3 phosphorylated on Tyrosine 1289.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB3 protein also known as HER3 is a member of the epidermal growth factor receptor (EGFR) family. It belongs to the ErbB receptor tyrosine kinase family but lacks intrinsic kinase activity. The HER3 protein has a molecular weight of approximately 180 kDa. You will find HER3 expressed in various tissues with high expression in the epithelial tissues where it plays a role in normal cellular processes. HER3 interacts with multiple ligands facilitating cellular communication and growth regulation.

Biological function summary

HER3 is essential for activating important signaling pathways that regulate proliferation differentiation and survival. The ErbB3 protein forms heterodimers with other ErbB family members such as ErbB2 (HER2) which is necessary for its signaling capability. The HER3 protein is abundant in heart and nervous tissues indicating its role in cardiovascular and neurological functions. Its activation modulates gene expression through downstream effectors mediated by dimerization.

Pathways

HER3 plays a pivotal role in the PI3K/Akt and MAPK signaling pathways. These pathways are fundamental in cellular responses like growth and survival. HER3 serves as a major partner for HER2 in these signaling cascades enhancing downstream signaling effects. HER3's connection to these pathways makes it a critical node collaborating closely with proteins such as HER2 and EGFR to maintain efficient communication and function within these networks.

HER3 associates strongly with cancer and cardiovascular diseases. Its overexpression in certain cancers such as breast cancer leads to enhanced tumor growth and resistance to therapy. HER3’s relationship with the HER2 protein is especially significant in HER2-positive breast cancer where their interaction drives tumor progression. Additionally alterations in HER3 expression or function impact cardiovascular diseases as it plays a significant role in heart and vascular cell behavior. Recognizing HER3's involvement in these conditions allows for targeted therapeutic approaches and improves disease management strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed : 20682778). May also be activated by CSPG5 (PubMed : 15358134). Involved in the regulation of myeloid cell differentiation (PubMed : 27416908).

See full target information ERBB3 phospho Y1289

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:299 PubMed40935839

2025

Coinhibition of the MEK/RTK pathway has high therapeutic efficacy in KRAS-mutant non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Lu,Minjuan Hu,Yikai Zhao,Tianqing Chu,Wei Zhang,Yijia Zhou,Xinlei Cai,Jun Wu,Liang Hu,Chunlei Shi,Liwen Xiong,Aiqin Gu,Huimin Wang,Yanwei Zhang,Yuqing Lou,Runbo Zhong,Zhiqiang Gao,Hongyu Liu,Chao Zhou,Yingli Wu,Liang Zhu,Hua Zhong,Hongbin Ji,Baohui Han

Scientific reports 15:16964 PubMed40374836

2025

Modulation of HER2 internalization enhances single-dose antibody-drug potency in HER2 gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Abbey Zidel,Alex Benton,Emma Brown,Shayla Shmuel,Alex Vanover,Sandeep Surendra Panikar,Ron Bose,Haeseong Park,Andrew A Davis,Patrícia M R Pereira

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 64:1638-1646 PubMed37385676

2023

Immuno-PET Detects Antibody-Drug Potency on Coadministration with Statins.

Applications

Unspecified application

Species

Unspecified reactive species

Emma L Brown,Shayla Shmuel,Komal Mandleywala,Sandeep Surendra Panikar,Na-Keysha Berry,Yi Rao,Abbey Zidel,Jason S Lewis,Patrícia M R Pereira

Acta biochimica Polonica 70:261-269 PubMed37331014

2023

β-catenin promotes resistance to trastuzumab in breast cancer cells through enhancing interaction between HER2 and SRC.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Hao,Jialu Zheng,Xiaoqin Yu,Zhixin Li,Guanghui Ren

Nature communications 13:2526 PubMed35534471

2022

Caveolin-1 temporal modulation enhances antibody drug efficacy in heterogeneous gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Patrícia M R Pereira,Komal Mandleywala,Sébastien Monette,Melissa Lumish,Kathryn M Tully,Sandeep Surendra Panikar,Mike Cornejo,Audrey Mauguen,Ashwin Ragupathi,Nai C Keltee,Marissa Mattar,Yelena Y Janjigian,Jason S Lewis

Molecular pharmaceutics 17:327-337 PubMed31804840

2019

HER2-Targeted PET Imaging and Therapy of Hyaluronan-Masked HER2-Overexpressing Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia M R Pereira,Ashwin Ragupathi,Shayla Shmuel,Komal Mandleywala,Nerissa T Viola,Jason S Lewis

Oncotarget 8:107423-107440 PubMed29296175

2017

Organotypic three-dimensional cancer cell cultures mirror drug responses : lessons learned from the inhibition of EGFR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nico Jacobi,Rita Seeboeck,Elisabeth Hofmann,Helmut Schweiger,Veronika Smolinska,Thomas Mohr,Alexandra Boyer,Wolfgang Sommergruber,Peter Lechner,Corina Pichler-Huebschmann,Kamil Önder,Harald Hundsberger,Christoph Wiesner,Andreas Eger

Oncogene 35:1554-64 PubMed26073080

2015

ErbB3 upregulation by the HNSCC 3D microenvironment modulates cell survival and growth.

Applications

Unspecified application

Species

Unspecified reactive species

J O Humtsoe,E Pham,R J Louie,D A Chan,R H Kramer

Cellular signalling 26:70-82 PubMed24036211

2013

HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4).

Applications

Unspecified application

Species

Human

James J Sollome,Elangovan Thavathiru,Todd D Camenisch,Richard R Vaillancourt

PloS one 7:e40243 PubMed22792252

2012

Hypoxia changes the expression of the epidermal growth factor (EGF) system in human hearts and cultured cardiomyocytes.

Applications

Species

Mouse

Mathias Munk,Ashfaque Ahmed Memon,Jens Peter Goetze,Lars Bo Nielsen,Ebba Nexo,Boe Sandahl Sorensen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325]

Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (AB76469)

Western blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (AB76469)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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