AB323861

Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19]

BOND RX™ Validated
RabMAb
Recombinant
20ul selling size
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal ErbB3 / HER3 phospho Y1289 antibody. Suitable for Dot, IHC-P and reacts with Synthetic peptide - Human, Human samples.

View Alternative Names

HER3, ERBB3, Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ErbB3 / HER3 (phospho Y1289) with ab323861 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human breast cancer without alkaline phosphatase treatment; No signal was detected when tissues were treated with alkaline phosphatase.
The section was incubated with for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ErbB3 / HER3 (phospho Y1289) with ab323861 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in human cerebrum.
The section was incubated with for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ErbB3 / HER3 (phospho Y1289) with ab323861 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in human liver.
The section was incubated with for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861)

Immunohistochemical analysis of paraffin-embedded (A) Untreated MCF7 starved overnight (without alkaline phosphatase treatment); (B) MCF7 (human breast adenocarcinoma epithelial cell) starved overnight, then treated with 100ng/ml Neuregulin-1 for 5min (without alkaline phosphatase treatment); (C) MCF7 starved overnight, then treated with 100ng/ml Neuregulin-1 for 5min (treated with alkaline phosphatase); (D) Untreated MCF7 starved overnight (treated with alkaline phosphatase) labeling ErbB3 / HER3 (phospho Y1289) with ab323861 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (B) : MCF7 starved overnight, then treated with 100ng/ml Neuregulin-1 for 5min (without alkaline phosphatase treatment) and weak staining in (A) : Untreated MCF7 starved overnight (without alkaline phosphatase treatment). No signal was detected when cell pellets were treated with alkaline phosphatase.
The section was incubated with for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Dot Blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861)

Dot blot analysis of ErbB3 / HER3 (phospho Y1289) using ab323861 at 1 : 1000 (0.488 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : ErbB3 / HER3 (phospho Y1289) peptide a
Lane 2 : ErbB3 / HER3 (phospho Y1289) peptide b
Lane 3 : ErbB3 / HER3 non-phospho peptide

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861) at 1/1000 dilution

Lane 1:

ErbB3 / HER3 (phospho Y1289) peptide a

Lane 2:

ErbB3 / HER3 (phospho Y1289) peptide b

Lane 3:

ErbB3 / HER3 non-phospho peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28708-19

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Cross reactivity to family members is not possible due to immunogen design.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed : 20682778). May also be activated by CSPG5 (PubMed : 15358134). Involved in the regulation of myeloid cell differentiation (PubMed : 27416908).

See full target information ERBB3 pY1289

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com