Skip to main content

Rabbit Recombinant Monoclonal ErbB3 / HER3 phospho Y1289 antibody. Suitable for Dot, IHC-P and reacts with Synthetic peptide - Human, Human samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861), expandable thumbnail
  • Dot Blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (AB323861), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIHC-P
Human
Expected
Tested
Mouse
Not recommended
Not recommended
Synthetic peptide - Human
Tested
Not recommended

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Synthetic peptide - Human
Dilution info
-
Notes

-

Target data

Function

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). May also be activated by CSPG5 (PubMed:15358134). Involved in the regulation of myeloid cell differentiation (PubMed:27416908).

Alternative names

HER3, ERBB3, Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3

Rabbit Recombinant Monoclonal ErbB3 / HER3 phospho Y1289 antibody. Suitable for Dot, IHC-P and reacts with Synthetic peptide - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28708-19
Purification technique
Affinity purification Protein A
Specificity

Cross reactivity to family members is not possible due to immunogen design.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861)

    ErbB3 / HER3 (phospho Y1289) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ErbB3 / HER3 (phospho Y1289) antibody

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ErbB3 / HER3 (phospho Y1289) with ab323861 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human breast cancer without alkaline phosphatase treatment; No signal was detected when tissues were treated with alkaline phosphatase.

    The section was incubated with for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861)

    ErbB3 / HER3 (phospho Y1289) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ErbB3 / HER3 (phospho Y1289) antibody

    Immunohistochemical analysis of paraffin-embedded (A) Untreated MCF7 starved overnight (without alkaline phosphatase treatment); (B) MCF7 (human breast adenocarcinoma epithelial cell) starved overnight, then treated with 100ng/ml Neuregulin-1 for 5min (without alkaline phosphatase treatment); (C) MCF7 starved overnight, then treated with 100ng/ml Neuregulin-1 for 5min (treated with alkaline phosphatase); (D) Untreated MCF7 starved overnight (treated with alkaline phosphatase) labeling ErbB3 / HER3 (phospho Y1289) with ab323861 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in (B): MCF7 starved overnight, then treated with 100ng/ml Neuregulin-1 for 5min (without alkaline phosphatase treatment) and weak staining in (A): Untreated MCF7 starved overnight (without alkaline phosphatase treatment). No signal was detected when cell pellets were treated with alkaline phosphatase.

    The section was incubated with for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861)

    ErbB3 / HER3 (phospho Y1289) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-ErbB3 / HER3 (phospho Y1289) antibody

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ErbB3 / HER3 (phospho Y1289) with ab323861 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining in human cerebrum.

    The section was incubated with for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861)

    ErbB3 / HER3 (phospho Y1289) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human liver using rabbit Anti-ErbB3 / HER3 (phospho Y1289) antibody

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ErbB3 / HER3 (phospho Y1289) with ab323861 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining in human liver.

    The section was incubated with for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Dot Blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861), expandable thumbnail

    Dot Blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861)

    ErbB3 / HER3 (phospho Y1289) Dot Blot staining using rabbit Anti-ErbB3 / HER3 (phospho Y1289) antibody

    Dot blot analysis of ErbB3 / HER3 (phospho Y1289) using ab323861 at 1:1000 (0.488 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane 1: ErbB3 / HER3 (phospho Y1289) peptide a

    Lane 2: ErbB3 / HER3 (phospho Y1289) peptide b

    Lane 3: ErbB3 / HER3 non-phospho peptide

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Dot Blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR28708-19] (ab323861) at 1/1000 dilution

    Lane 1: ErbB3 / HER3 (phospho Y1289) peptide a

    Lane 2: ErbB3 / HER3 (phospho Y1289) peptide b

    Lane 3: ErbB3 / HER3 non-phospho peptide

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com