AB131444

Anti-ErbB3 / HER3 (phospho Y1328) antibody

Name: Anti-ErbB3 / HER3 (phospho Y1328) antibody (ab131444) | Abcam
Brand: Abcam Limited
SKU: AB131444
Price: 545 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal ErbB3 / HER3 phospho Y1328 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ERBB3 pY1328 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HER3, ERBB3, Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1328) antibody (AB131444)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1328) antibody (AB131444)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling ErbB3 / HER3 (phospho Y1328) with ab131444 antibody at 1/50 dilution. The image on the right is treated with the synthesized peptide.

Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 (phospho Y1328) antibody (AB131444)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 (phospho Y1328) antibody (AB131444)

Immunofluorescence of methanol-fixed MCF7 cells labelling EErbB3 / HER3 (phospho Y1328) with ab131444 antibody at 1/100 dilution.

Unknown

Western blot - Anti-ErbB3 / HER3 (phospho Y1328) antibody (AB131444)

All lanes:

Western blot - Anti-ErbB3 / HER3 (phospho Y1328) antibody (ab131444) at 1/500 dilution

Lane 1:

A431 cell extract, untreated

Lane 2:

A431 cell extract, treated with EGF

Predicted band size: 148 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ERBB3 pY1328 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P21860

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab131444 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB3 protein also known as HER3 is a member of the epidermal growth factor receptor (EGFR) family. It belongs to the ErbB receptor tyrosine kinase family but lacks intrinsic kinase activity. The HER3 protein has a molecular weight of approximately 180 kDa. You will find HER3 expressed in various tissues with high expression in the epithelial tissues where it plays a role in normal cellular processes. HER3 interacts with multiple ligands facilitating cellular communication and growth regulation.

Biological function summary

HER3 is essential for activating important signaling pathways that regulate proliferation differentiation and survival. The ErbB3 protein forms heterodimers with other ErbB family members such as ErbB2 (HER2) which is necessary for its signaling capability. The HER3 protein is abundant in heart and nervous tissues indicating its role in cardiovascular and neurological functions. Its activation modulates gene expression through downstream effectors mediated by dimerization.

Pathways

HER3 plays a pivotal role in the PI3K/Akt and MAPK signaling pathways. These pathways are fundamental in cellular responses like growth and survival. HER3 serves as a major partner for HER2 in these signaling cascades enhancing downstream signaling effects. HER3's connection to these pathways makes it a critical node collaborating closely with proteins such as HER2 and EGFR to maintain efficient communication and function within these networks.

HER3 associates strongly with cancer and cardiovascular diseases. Its overexpression in certain cancers such as breast cancer leads to enhanced tumor growth and resistance to therapy. HER3’s relationship with the HER2 protein is especially significant in HER2-positive breast cancer where their interaction drives tumor progression. Additionally alterations in HER3 expression or function impact cardiovascular diseases as it plays a significant role in heart and vascular cell behavior. Recognizing HER3's involvement in these conditions allows for targeted therapeutic approaches and improves disease management strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed : 20682778). May also be activated by CSPG5 (PubMed : 15358134). Involved in the regulation of myeloid cell differentiation (PubMed : 27416908).

See full target information ERBB3 pY1328

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 14:1543-1555 PubMed37073425

2023

Downregulation of circ_PLXND1 inhibits tumorigenesis of non-small cell lung carcinoma via miR-1287-5p/ERBB3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinzhou Wu,Chenyang Liu,Guiping Yu

Molecular biology of the cell 32:1838-1848 PubMed34260260

2021

p52Shc regulates the sustainability of ERK activation in a RAF-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Yoshizawa,Nobuhisa Umeki,Akihiro Yamamoto,Mariko Okada,Masayuki Murata,Yasushi Sako

Biophysics and physicobiology 18:1-12 PubMed33665062

2021

Biphasic spatiotemporal regulation of GRB2 dynamics by p52SHC for transient RAS activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Yoshizawa,Nobuhisa Umeki,Akihiro Yamamoto,Masayuki Murata,Yasushi Sako

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ErbB3 / HER3 (phospho Y1328) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 (phospho Y1328) antibody (AB131444)

Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 (phospho Y1328) antibody (AB131444)

Western blot - Anti-ErbB3 / HER3 (phospho Y1328) antibody (AB131444)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise