AB32375

Anti-ErbB4 / HER4 antibody [E200]

Name: Anti-ErbB4 / HER4 antibody [E200] (ab32375) | Abcam
Brand: Abcam Limited
SKU: AB32375
Price: 365 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(2 Reviews)

(35 Publications)

Anti-ErbB4 / HER4 antibody [E200] (ab32375) is a rabbit monoclonal antibody detecting ErbB4 / HER4 in Western Blot, IP. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
- Trusted since 2006

View Alternative Names

HER4, ERBB4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4

4 Images

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Immunoprecipitation - Anti-ErbB4 / HER4 antibody [E200] (AB32375)

Lane 1 (input) : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate, 10μg
Lane 2 (+) : HEK-293 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32375 in HEK-293 whole cell lysate

ab32375 Immunoprecipitating ErbB 4 in HEK-293 whole cell lysate. Capture antibody was used at a 1 : 50 dilution (2μg in 0.35mg lysates). For western blotting, primary antibody used as ab32375 at 1 : 500 dilution. ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection at 1 : 1000 dilution. The lower band at around 75kDa should be proteolysis fragment based on the literature. (PMID : 9362517)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-ErbB4 / HER4 antibody [E200] (ab32375)

Predicted band size: 147 kDa

Observed band size: 185 kDa

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Exposure time: 30s

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Western blot - Anti-ErbB4 / HER4 antibody [E200] (AB32375)

All lanes:

Western blot - Anti-ErbB4 / HER4 antibody [E200] (ab32375) at 1/4000 dilution

All lanes:

MCF7 cell lysate

Predicted band size: 147 kDa

Observed band size: 185 kDa

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Unknown

Western blot - Anti-ErbB4 / HER4 antibody [E200] (AB32375)

All lanes:

Western blot - Anti-ErbB4 / HER4 antibody [E200] (ab32375) at 1.09 µg/mL

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) at 20 µg

Lane 2:

T47D (Human ductal breast epithelial tumor epithelial cell) at 20 µg

Lane 3:

T47D (Human ductal breast epithelial tumor epithelial cell) low exposure image of lane 2 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 147 kDa

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Lab

Western blot - Anti-ErbB4 / HER4 antibody [E200] (AB32375)

Western blot : Anti-ErbB4 / HER4 antibody [E200] ab32375 staining at 1/2000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 171 kDa in T-47D cell lysates with no signal observed at this size in A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 100 % LICOR in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ErbB4 / HER4 antibody [E200] (ab32375) at 1/2000 dilution

Lane 1:

T-47D at 40 µg

Lane 2:

A549 at 40 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 171 kDa

Observed band size: 171 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E200

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to ErbB4 / HER4. It does not cross react with other EGF receptor family members.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-ErbB4 / HER4 antibody [E200] (ab32375) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human samples.

What is the molecular weight of ErbB4 / HER4?
Anti-ErbB4 / HER4 [E200] (ab32375) specifically detects a band for ErbB4 / HER4 (UniProt: Q15303) at a molecular weight of 147kDa.

Trusted by the scientific community
Anti-ErbB4 / HER4 [E200] (ab32375) was first used in a scientific publication in 2006 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [E200] also available for your convenience: ab32375, Carrier free - ab183299

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ErbB4 also known as HER4 is a member of the ErbB family of receptor tyrosine kinases. It functions mechanically as a transmembrane protein that activates downstream signaling pathways upon ligand binding. This receptor has an approximate mass of 180 kDa. You can find ErbB4 mainly expressed in the heart brain and various epithelial tissues. The ErbB4 protein often interacts with ligands such as Neuregulins heregulin and others.

Biological function summary

ErbB4 has several roles including the regulation of cell proliferation differentiation migration and development. It functions as part of a receptor complex initiating a cascade of intracellular signaling that impacts various cellular functions. ErbB4 ELISA assays often help quantify interactions and activities of the receptor providing insights into its broader functionalities. The ErbB4 protein also contributes to neuronal development and the maintenance of cardiac tissue integrity.

Pathways

ErbB4 participates in the PI3K/AKT pathway and the MAPK/ERK pathway essential for cell survival and proliferation. These pathways involve a network of proteins including other ErbB family members such as EGFR ErbB2 (HER2) and ErbB3 (HER3) forming heterodimers that enhance and diversify the signaling outcomes. E200 and HF R are often referred to in literature related to pathway analysis of these complex interactions.

ErbB4 has links to several conditions notably certain cancers and schizophrenia. The connection to cancer arises due to its role in oncogenic signaling where it can form aberrant partnerships with proteins like ErbB2 which stimulates cell growth. In schizophrenia alterations in ErbB4 expression or function may affect neural signaling pathways leading to cognitive deficits. Understanding these relationships helps to target therapies and develop treatments involving anti HER HF R 500 and similar inhibitors.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.

See full target information ERBB4

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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39000582

2024

HER4 Affects Sensitivity to Tamoxifen and Abemaciclib in Luminal Breast Cancer Cells and Restricts Tumor Growth in MCF-7-Based Humanized Tumor Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Veruschka Albert,Christina Bruss,Deniz Tümen,Gerhard Piendl,Florian Weber,Edgar Dahl,Stephan Seitz,Olaf Ortmann,Anja K Wege,Gero Brockhoff

iScience 27:108839 PubMed38303712

2024

Profiling of ERBB receptors and downstream pathways reveals selectivity and hidden properties of ERBB4 antagonists.

Applications

Unspecified application

Species

Unspecified reactive species

Lukša Popović,Jan P Wintgens,Yuxin Wu,Ben Brankatschk,Sascha Menninger,Carsten Degenhart,Niels Jensen,Sven P Wichert,Bert Klebl,Moritz J Rossner,Michael C Wehr

EMBO reports 24:e56689 PubMed37009825

2023

STAT5b is a key effector of NRG-1/ERBB4-mediated myocardial growth.

Applications

Unspecified application

Species

Unspecified reactive species

Katri Vaparanta,Anne Jokilammi,Ilkka Paatero,Johannes A Merilahti,Juho Heliste,Karthik Amudhala Hemanthakumar,Riikka Kivelä,Kari Alitalo,Pekka Taimen,Klaus Elenius

Nature metabolism 4:1573-1590 PubMed36400933

2022

Brown adipose tissue-derived Nrg4 alleviates endothelial inflammation and atherosclerosis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lingfeng Shi,Yixiang Li,Xiaoli Xu,Yangyang Cheng,Biying Meng,Jinling Xu,Lin Xiang,Jiajia Zhang,Kaiyue He,Jiayue Tong,Junxia Zhang,Lingwei Xiang,Guangda Xiang

iScience 25:104407 PubMed35663028

2022

Neuregulin-1/ErbB4 signaling modulates HRP2-induced damage to brain cortical organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Harbuzariu,Annette Nti,Keri Oxendine Harp,Juan C Cespedes,Adel Driss,Jonathan K Stiles

Frontiers in endocrinology 13:812032 PubMed35370955

2022

MOTS-c and Exercise Restore Cardiac Function by Activating of NRG1-ErbB Signaling in Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shunchang Li,Manda Wang,Jiacheng Ma,Xiaoli Pang,Jinghan Yuan,Yanrong Pan,Yu Fu,Ismail Laher

Molecular biology reports 49:3975-3986 PubMed35166983

2022

Neuregulin-1 regulates the conversion of M1/M2 microglia phenotype via ErbB4-dependent inhibition of the NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Ma,Peixia Fan,Rui Zhao,Yinghua Zhang,Xianwei Wang,Weigang Cui

Cancer research communications 2:10-27 PubMed36860695

2022

An Unbiased Functional Genetics Screen Identifies Rare Activating ERBB4 Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Deepankar Chakroborty,Veera K Ojala,Anna M Knittle,Jasmin Drexler,Mahlet Z Tamirat,Regina Ruzicka,Karin Bosch,Johanna Woertl,Susanne Schmittner,Laura L Elo,Mark S Johnson,Kari J Kurppa,Flavio Solca,Klaus Elenius

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 47:1292-1303 PubMed34707248

2021

ErbB4 in parvalbumin-positive interneurons mediates proactive interference in olfactory associative reversal learning.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Xu,Meng-Lin Wang,Hui Tao,Chi Geng,Feng Guo,Bin Hu,Ran Wang,Xiao-Yu Hou

The Journal of comparative neurology 529:3497-3512 PubMed34212389

2021

ErbB4 mediates amyloid β-induced neurotoxicity through JNK/tau pathway activation: Implications for Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhang,Ling Zhang,Dongming Zhou,Hongfei Li,Yang Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ErbB4 / HER4 antibody [E200]

Immunoprecipitation - Anti-ErbB4 / HER4 antibody [E200] (AB32375)

Western blot - Anti-ErbB4 / HER4 antibody [E200] (AB32375)

Western blot - Anti-ErbB4 / HER4 antibody [E200] (AB32375)

Western blot - Anti-ErbB4 / HER4 antibody [E200] (AB32375)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (35)

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