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AB247386

Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ErbB4 / HER4 phospho Y1162 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HER4, ERBB4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (AB247386)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (AB247386)

This data was developed using ab68478 the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ErbB4 / HER4 with ab68478 at 1/1000 dilution, followed by ab209101 Rabbit specific IHC polymer detection kit HRP/DAB. Positive staining on Human breast carcinoma without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.

Immunoprecipitation - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (AB247386)
  • IP

Supplier Data

Immunoprecipitation - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (AB247386)

This data was developed using ab68478 the same antibody clone in a different buffer. ErbB4 / HER4 (phospho Y1162) was immunoprecipitated from 0.35 mg of A431 treated with EGF (100ng/ml 30min) whole cell lysate with ab68478 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab68478 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

All lanes:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate

false

Western blot - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (AB247386)
  • WB

Supplier Data

Western blot - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (AB247386)

This data was developed using ab68478 the same antibody clone in a different buffer. Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (<a href='/en-us/products/primary-antibodies/erbb4-her4-phospho-y1162-antibody-ep2270y-ab68478'>ab68478</a>) at 1/5000 dilution

Lane 1:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) treated with 100 ng/ml EGF for 30 min whole cell lysate

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) treated with 100 ng/ml EGF for 30 min whole cell lysate, then membrane treated with Alkaline phosphatase

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 200 kDa

false

Exposure time: 10s

  • Unconjugated

    Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2270Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

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Product details

ab247386 is the carrier-free version of ab68478.

This product has switched from a hybridoma to recombinant production method on 31st May 2023.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ErbB4 also known as HER4 is a member of the ErbB family of receptor tyrosine kinases. It functions mechanically as a transmembrane protein that activates downstream signaling pathways upon ligand binding. This receptor has an approximate mass of 180 kDa. You can find ErbB4 mainly expressed in the heart brain and various epithelial tissues. The ErbB4 protein often interacts with ligands such as Neuregulins heregulin and others.
Biological function summary

ErbB4 has several roles including the regulation of cell proliferation differentiation migration and development. It functions as part of a receptor complex initiating a cascade of intracellular signaling that impacts various cellular functions. ErbB4 ELISA assays often help quantify interactions and activities of the receptor providing insights into its broader functionalities. The ErbB4 protein also contributes to neuronal development and the maintenance of cardiac tissue integrity.

Pathways

ErbB4 participates in the PI3K/AKT pathway and the MAPK/ERK pathway essential for cell survival and proliferation. These pathways involve a network of proteins including other ErbB family members such as EGFR ErbB2 (HER2) and ErbB3 (HER3) forming heterodimers that enhance and diversify the signaling outcomes. E200 and HF R are often referred to in literature related to pathway analysis of these complex interactions.

ErbB4 has links to several conditions notably certain cancers and schizophrenia. The connection to cancer arises due to its role in oncogenic signaling where it can form aberrant partnerships with proteins like ErbB2 which stimulates cell growth. In schizophrenia alterations in ErbB4 expression or function may affect neural signaling pathways leading to cognitive deficits. Understanding these relationships helps to target therapies and develop treatments involving anti HER HF R 500 and similar inhibitors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.
See full target information ERBB4 pY1162
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 18:477 PubMed37393232

2023

CircTBX5 knockdown modulates the miR-558/MyD88 axis to alleviate IL-1β-induced inflammation, apoptosis and extracellular matrix degradation in chondrocytes via inactivating the NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Hongjie Mu,Qiaoyi Cui,Peng Yu,Tong Liu,Tao Wang,Lin Sheng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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