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AB109273

Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)]

5

(1 Review)

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(10 Publications)

Rabbit Recombinant Monoclonal ErbB4 / HER4 phospho Y1284 antibody. Suitable for WB, Dot and reacts with Human, Synthetic peptide samples. Cited in 10 publications.

View Alternative Names

HER4, ERBB4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4

3 Images
Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (AB109273)
  • WB

Supplier Data

Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (AB109273)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273) at 1/1000 dilution

Lane 1:

Untreated A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

A431 treated with 100 ng/ml Epidermal Growth Factor (EGF) for 30 min whole cell lysate at 15 µg

Lane 3:

A431 treated with 100 ng/ml Epidermal Growth Factor (EGF) for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 147 kDa

Observed band size: 180 kDa

false

Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (AB109273)
  • WB

Supplier Data

Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (AB109273)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273) at 1/1000 dilution

Lane 1:

Untreated A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

A431 treated with 50mM pervanadate for 5 min whole cell lysate at 15 µg

Lane 3:

A431 treated with 50mM pervanadate for 5 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 147 kDa

Observed band size: 180 kDa

false

Dot Blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (AB109273)
  • Dot

Supplier Data

Dot Blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (AB109273)

Dot blot analysis using 1/1000 dilution ab109273 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary at 1/100000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lane 1 : ErbB4 non-phospho peptide

Lane 2 : ErbB4 Y1284 phospho peptide

Lane 3 : ErbB2 non-phospho peptide

Lane 4 : ErbB2 Y1248 phospho peptide

Exposure time : 3 minutes

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2273(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects ErbB4 / HER4 phosphorylated at Tyrosine 1284 and may also detect Erbb2/HER2 phosphorylated at Tyrosine 1248.

Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ErbB4 also known as HER4 is a member of the ErbB family of receptor tyrosine kinases. It functions mechanically as a transmembrane protein that activates downstream signaling pathways upon ligand binding. This receptor has an approximate mass of 180 kDa. You can find ErbB4 mainly expressed in the heart brain and various epithelial tissues. The ErbB4 protein often interacts with ligands such as Neuregulins heregulin and others.
Biological function summary

ErbB4 has several roles including the regulation of cell proliferation differentiation migration and development. It functions as part of a receptor complex initiating a cascade of intracellular signaling that impacts various cellular functions. ErbB4 ELISA assays often help quantify interactions and activities of the receptor providing insights into its broader functionalities. The ErbB4 protein also contributes to neuronal development and the maintenance of cardiac tissue integrity.

Pathways

ErbB4 participates in the PI3K/AKT pathway and the MAPK/ERK pathway essential for cell survival and proliferation. These pathways involve a network of proteins including other ErbB family members such as EGFR ErbB2 (HER2) and ErbB3 (HER3) forming heterodimers that enhance and diversify the signaling outcomes. E200 and HF R are often referred to in literature related to pathway analysis of these complex interactions.

ErbB4 has links to several conditions notably certain cancers and schizophrenia. The connection to cancer arises due to its role in oncogenic signaling where it can form aberrant partnerships with proteins like ErbB2 which stimulates cell growth. In schizophrenia alterations in ErbB4 expression or function may affect neural signaling pathways leading to cognitive deficits. Understanding these relationships helps to target therapies and develop treatments involving anti HER HF R 500 and similar inhibitors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.
See full target information ERBB4 phospho Y1284
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmacologica Sinica 45:1287-1304 PubMed38360930

2024

Quantitative systems pharmacology modeling of HER2-positive metastatic breast cancer for translational efficacy evaluation and combination assessment across therapeutic modalities.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ting Zhou,Jia-Hui Chu,Shu-Han Zhao,Ge-Li Li,Zi-Yi Fu,Su-Jie Zhang,Xue-Hu Gao,Wen Ma,Kai Shen,Yuan Gao,Wei Li,Yong-Mei Yin,Chen Zhao

Molecular biology reports 49:3975-3986 PubMed35166983

2022

Neuregulin-1 regulates the conversion of M1/M2 microglia phenotype via ErbB4-dependent inhibition of the NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Ma,Peixia Fan,Rui Zhao,Yinghua Zhang,Xianwei Wang,Weigang Cui

Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer 40:61-72 PubMed34936301

2021

Targeting Neuregulin 1 (NRG1): A Novel Biomarker for Non-Small-Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Fang,Baoguo Kang,Pan Zhao,Jing Ran,Lifang Wang,Lingqiong Zhao,Hangyu Luo,Ling Tao

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:9872-9890 PubMed34725188

2021

Sustained ErbB Activation Causes Demyelination and Hypomyelination by Driving Necroptosis of Mature Oligodendrocytes and Apoptosis of Oligodendrocyte Precursor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Hu,Guanxiu Xiao,Li He,Xiaojie Niu,Huashun Li,Tianjie Lou,Qianqian Hu,Youguang Yang,Qi Xu,Zhengdong Wei,Mengsheng Qiu,Kenji F Tanaka,Ying Shen,Yanmei Tao

Nature communications 10:1670 PubMed30975989

2019

Multi-region sequencing unveils novel actionable targets and spatial heterogeneity in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Yan,Heyang Cui,Yong Zhou,Bin Yang,Pengzhou Kong,Yingchun Zhang,Yiqian Liu,Bin Wang,Yikun Cheng,Jiayi Li,Shixing Guo,Enwei Xu,Huijuan Liu,Caixia Cheng,Ling Zhang,Ling Chen,Xiaofei Zhuang,Yu Qian,Jian Yang,Yanchun Ma,Hongyi Li,Fang Wang,Jing Liu,Xuefeng Liu,Dan Su,Yan Wang,Ruifang Sun,Shiping Guo,Yaoping Li,Xiaolong Cheng,Zhihua Liu,Qimin Zhan,Yongping Cui

Journal of neuroinflammation 15:104 PubMed29636063

2018

Neuregulin-1 attenuates experimental cerebral malaria (ECM) pathogenesis by regulating ErbB4/AKT/STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mingli Liu,Wesley Solomon,Juan Carlos Cespedes,Nana O Wilson,Byron Ford,Jonathan K Stiles

Cell discovery 3:17044 PubMed29238610

2017

A role for ErbB signaling in the induction of reactive astrogliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Wanwan He,Xu Hu,Yuwen Shen,Junyan Cao,Zhengdong Wei,Yifei Luan,Li He,Fangdun Jiang,Yanmei Tao

The Journal of clinical investigation 126:4444-4459 PubMed27775552

2016

Transcription factor ETV1 is essential for rapid conduction in the heart.

Applications

IHC-Fr

Species

Mouse

Akshay Shekhar,Xianming Lin,Fang-Yu Liu,Jie Zhang,Huan Mo,Lisa Bastarache,Joshua C Denny,Nancy J Cox,Mario Delmar,Dan M Roden,Glenn I Fishman,David S Park

Cell death & disease 6:e1765 PubMed25996292

2015

Activation of NRG1-ERBB4 signaling potentiates mesenchymal stem cell-mediated myocardial repairs following myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

X Liang,Y Ding,Y Zhang,Y-H Chai,J He,S-M Chiu,F Gao,H-F Tse,Q Lian

Infection and immunity 82:1243-55 PubMed24379285

2014

Role of epidermal growth factor receptor signaling in the interaction of Neisseria meningitidis with endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Heiko Slanina,Sabrina Mündlein,Sabrina Hebling,Alexandra Schubert-Unkmeir
View all publications
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