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Rabbit Recombinant Monoclonal ErbB4 / HER4 antibody. Suitable for I-ELISA, WB, IHC-P and reacts with Recombinant fragment - Human, Mouse, Human samples. Cited in 2 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (AB219208), expandable thumbnail
  • Western blot - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (AB219208), expandable thumbnail
  • Indirect ELISA - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (AB219208), expandable thumbnail
  • Western blot - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (AB219208), expandable thumbnail
  • Indirect ELISA - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (AB219208), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPI-ELISAWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Expected
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Expected
Tested
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Not recommended
Tested
Tested
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.

Additional Targets

ErbB2 / HER2

Alternative names

HER4, ERBB4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4

Recommended products

Rabbit Recombinant Monoclonal ErbB4 / HER4 antibody. Suitable for I-ELISA, WB, IHC-P and reacts with Recombinant fragment - Human, Mouse, Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22665-104
Purification technique
Affinity purification Protein A
Specificity

ab219208 is not recommended for mouse IHC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ErbB2 also known as HER2 and ErbB4 also known as HER4 are members of the ErbB family of receptor tyrosine kinases. ErbB2 has a molecular mass of about 185 kDa and lacks a natural ligand while ErbB4 weighs around 180 kDa and does bind to specific ligands. These receptors are mainly expressed in epithelial tissues including those found in the gastrointestinal tract respiratory system and skin. These receptors function as transmembrane proteins playing an important role in signal transduction processes by phosphorylating specific tyrosine residues upon dimerization.

Biological function summary

ErbB2 and ErbB4 serve significant roles in cellular processes such as cell growth differentiation and survival. ErbB2 typically forms heterodimers with other ErbB family members as it does not have an identified ligand. ErbB4 can function in both homo- and heterodimers. These complexes transmit signals inside the cell to influence transcription and other cellular responses. Their involvement is important for normal development and maintenance of various tissues.

Pathways

ErbB2 and ErbB4 participate in the PI3K/Akt and MAPK signaling pathways which are essential for mediating cellular responses like proliferation and survival. In these pathways they are closely related to proteins such as PI3K and Akt which are vital for transmitting and amplifying the signals initiated by ErbB receptor activation. These pathways ensure appropriate cellular responses to external stimuli and maintain cellular homeostasis.

Associated diseases and disorders

ErbB2 is strongly connected to breast cancer especially in cases where it is overexpressed leading to aggressive tumor growth. HER2 proteins are often the target for treatments in such cancers. ErbB4 shows a different profile and has been linked to heart development issues and schizophrenia. Both receptors interact with various ligands and other ErbB proteins during disease progression influencing prognosis and treatment options. Their distinct roles in different tissues explain their varied involvement in diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ErbB4 / HER4 with ab219208 at 1/1000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the human breast carcinoma (PMID: 19239686) is observed. The section was incubated with ab219208 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Western blot - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208), expandable thumbnail

    Western blot - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22761786) Lane 4 was developed using a higher sensitivity ECL substrate.

    Exposure time: Lane 1:70 seconds; Lanes 2-3:48 seconds; Lane 4: 3 minutes.

    All lanes: Western blot - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208) at 1/1000 dilution

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

    Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 180 kDa

  • Indirect ELISA - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208), expandable thumbnail

    Indirect ELISA - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208)

    ELISA analysis of Human ERBB4 recombinant protein at 1000 ng/mL with ab219208. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Western blot - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208), expandable thumbnail

    Western blot - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208)

    Blocking buffer and concentration : 5% NFDM/TBST
    Diluting buffer and concentration : 5% NFDM /TBST

    All lanes: Western blot - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208) at 1/1000 dilution

    Lane 1: His-tagged Recombinant Human ErbB2 protein (aa676-1255)

    Lane 2: His-tagged Recombinant Human ErbB4 protein (aa676-1308)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 90 kDa, 81 kDa

    Exposure time: 180s

  • Indirect ELISA - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208), expandable thumbnail

    Indirect ELISA - Anti-ErbB4 / HER4+ErbB2 / HER2 antibody [EPR22665-104] (ab219208)

    Indirect ELISA analysis of Human ERBB2 Recombinant Protein and Human ERBB4 Recombinant Protein at varying antigen concentrations of 500-0 ng/ml using ab219208 at 1000 ng/mL and secondary antibody Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution. Substrate solution was 3,3’,5,5’-Tetramethylbenzidine (TMB).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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