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AB241948

Anti-Erbin antibody

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(1 Publication)

Rabbit Polyclonal ERBIN antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ERBIN aa 800-900.

View Alternative Names

ERBB2IP, KIAA1225, LAP2, ERBIN, Erbin, Densin-180-like protein, Erbb2-interacting protein, Protein LAP2

2 Images
Western blot - Anti-Erbin antibody (AB241948)
  • WB

Supplier Data

Western blot - Anti-Erbin antibody (AB241948)

All lanes:

Western blot - Anti-Erbin antibody (ab241948) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 158 kDa

true

Exposure time: 3min

Immunoprecipitation - Anti-Erbin antibody (AB241948)
  • IP

Supplier Data

Immunoprecipitation - Anti-Erbin antibody (AB241948)

Erbin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

ab241948 used for IP at 6 μg/mg lysate. For WB at 1 μg/ml.

Lane 1 : ab241948 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Erbin antibody (ab241948)

Predicted band size: 158 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ERBIN aa 800-900. The exact immunogen used to generate this antibody is proprietary information.

Q96RT1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to Erbin immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Erbin also known as ERBB2IP (ERBB2 interacting protein) is a scaffolding protein with a mass of approximately 200 kDa. This protein localizes primarily at cell-cell junctions and participates in the stabilization of the basolateral membrane in epithelial cells. Erbin contains a PDZ domain that facilitates its interactions with other proteins allowing it to function effectively in cell signaling. It is widely expressed in various tissues including the brain heart and liver which indicate its importance in different physiological contexts.
Biological function summary

Erbin plays a role in maintaining epithelial cell polarity and adherence. It is part of the leucine-rich repeat and PDZ domain (LAP) protein family and interacts with receptor tyrosine kinases particularly the ERBB family. Furthermore Erbin forms a complex with other proteins to modulate signaling pathways that control cell proliferation and differentiation. Through these interactions Erbin influences key cellular processes that are fundamental for tissue organization and stability.

Pathways

Erbin participates in the ERBB signaling and MAPK/ERK pathways. Through the ERBB signaling pathway Erbin interacts with ERBB2 a receptor tyrosine kinase to regulate downstream signaling that affects cell growth and survival. In the MAPK/ERK pathway Erbin contributes to signal transduction processes important for cellular responses to growth factors. These pathways rely on the precise modulation of signaling by proteins like Erbin to maintain normal cellular function and prevent aberrant cell proliferation.

Erbin has been implicated in cancers and cardiac disorders. Its interaction with ERBB2 links it to breast cancer where dysregulation of this signaling pathway can lead to uncontrolled cell growth. Additionally Erbin is associated with heart failure as changes in its expression or function affect cardiac cell structure and function. In these conditions Erbin's interactions with other proteins can influence disease progression by altering key signaling events important for maintaining normal tissue homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state (PubMed : 16203728). Inhibits NOD2-dependent NF-kappa-B signaling and pro-inflammatory cytokine secretion (PubMed : 16203728).
See full target information ERBIN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 31:747-751 PubMed38467876

2024

Structural basis of human U5 snRNP late biogenesis and recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Riabov Bassat,Supapat Visanpattanasin,Matthias K Vorländer,Laura Fin,Alexander W Phillips,Clemens Plaschka
View all publications
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