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AB236442

Anti-ERCC1 antibody [SP68] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ERCC1 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

DNA excision repair protein ERCC-1, ERCC1

14 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling ERCC1 with Purified ab225721 at 1/100 dilution (0.53 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human testis tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human colon tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling ERCC1 with Purified ab225721 at 1/400 dilution (0.132 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (AB236442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling ERCC1 with Purified ab225721 at 1/400 dilution (0.132 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP68

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab236442 is the carrier-free version of ab225721.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERCC1 also known as Excision Repair Cross-Complementation Group 1 is an essential protein in DNA repair mechanisms. Weighing approximately 36 kDa ERCC1 is expressed in a variety of tissues throughout an organism. It carries out its function by forming a heterodimer with XPF which is a structure-specific endonuclease playing a vital role in the nucleotide excision repair (NER) pathway that helps maintain genomic stability. The presence of an antibody like 8F1 can be helpful for detecting the ERCC1 protein expression in biological samples through techniques like ELISA.
Biological function summary

ERCC1 plays a significant role in the DNA damage repair process where it interacts with its partner protein XPF to excise damaged DNA. The ERCC1-XPF complex recognizes and cuts both single- and double-stranded DNA therefore enabling the removal of DNA lesions caused by UV radiation or chemical mutagens. This action prevents mutations by ensuring accurate DNA replication and transcription.

Pathways

The ERCC1 protein is primarily involved in the nucleotide excision repair (NER) and interstrand cross-link repair pathways. Within these pathways ERCC1 provides repair capacity to the cell maintaining the functionality of the DNA by partnering with proteins such as XPF and participating in cross-link repair mechanisms. By coordinating with proteins like XPA and RPA ERCC1 assists in identifying and binding the damaged DNA facilitating the recruitment of other repair proteins.

The ERCC1 protein is linked to conditions such as Xeroderma Pigmentosum and Fanconi Anemia largely due to its critical role in DNA repair. Malfunctions or deficiencies in ERCC1 or its partner proteins can lead to compromised DNA repair resulting in susceptibility to cancer and other genomic instability disorders. ERCC1's relationship with proteins that partake in cell cycle regulation further emphasizes its impact on disease progression linking it with potential therapeutic targets for addressing these genetic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.. Isoform 2. Not functional in the nucleotide excision repair pathway.. Isoform 3. Not functional in the nucleotide excision repair pathway.. Isoform 4. Not functional in the nucleotide excision repair pathway.
See full target information ERCC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:3399 PubMed35697697

2022

Gene expression signatures of individual ductal carcinoma in situ lesions identify processes and biomarkers associated with progression towards invasive ductal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Clare A Rebbeck,Jian Xian,Susanne Bornelöv,Joseph Geradts,Amy Hobeika,Heather Geiger,Jose Franco Alvarez,Elena Rozhkova,Ashley Nicholls,Nicolas Robine,Herbert K Lyerly,Gregory J Hannon
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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