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AB327230

Anti-ERCC3 Antibody

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Rabbit Polyclonal XPB antibody. Suitable for ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ERCC3.

View Alternative Names

XPB, XPBC, ERCC3, General transcription and DNA repair factor IIH helicase/translocase subunit XPB, TFIIH subunit XPB, Basic transcription factor 2 89 kDa subunit, DNA 3'-5' helicase/translocase XPB, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa subunit, Xeroderma pigmentosum group B-complementing protein, BTF2 p89, TFIIH 89 kDa subunit, TFIIH p89

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ERCC3 Antibody (AB327230)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERCC3 Antibody (AB327230)

Immunofluorescent staining of human cell line A-431 using ab327230 at 0.25–2 µg/ml, showing localization to nucleoplasm.

ChIP - Anti-ERCC3 Antibody (AB327230)
  • ChIP

Supplier Data

ChIP - Anti-ERCC3 Antibody (AB327230)

ChIP-Exo-Seq composite graph for ab327230 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human ERCC3.

P19447

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent 3'-5' DNA helicase/translocase (PubMed : 17466626, PubMed : 27193682, PubMed : 33902107, PubMed : 8465201, PubMed : 8663148). Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity (PubMed : 27193682, PubMed : 33902107). Component of the general transcription and DNA repair factor IIH (TFIIH) core complex (PubMed : 10024882, PubMed : 17466626, PubMed : 8157004, PubMed : 8465201). When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening; it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor (PubMed : 10024882, PubMed : 17466626). In transcription, TFIIH has an essential role in transcription initiation (PubMed : 30894545, PubMed : 8157004). When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape (PubMed : 8157004). The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape (PubMed : 10024882). In transcription pre-initiation complexes induces and propagates a DNA twist to open DNA (PubMed : 27193682, PubMed : 33902107). Also involved in transcription-coupled nucleotide excision repair (NER) of damaged DNA (PubMed : 17466626, PubMed : 2111438, PubMed : 8157004). In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex; large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA (PubMed : 31253769, PubMed : 33902107). XPA retains XPB/ERCC3 at the 5' end of a DNA bubble (mimicking DNA damage) (PubMed : 31253769).
See full target information ERCC3
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Product promise

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