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AB275596

Anti-ERCC8 antibody

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Rabbit Polyclonal ERCC8 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human ERCC8.

View Alternative Names

CKN1, CSA, ERCC8, DNA excision repair protein ERCC-8, Cockayne syndrome WD repeat protein CSA

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ERCC8 antibody (AB275596)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERCC8 antibody (AB275596)

Immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ERCC8 with ab275596 at 1/1000 dilution at 4°C overnight, followed by Goat Anti-rabbit IgG Alexa Fluor®594-conjugated secondary antibody (Red). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS and blocked with 10% serum prior incubation. Positive staining was localized to nucleus.

Western blot - Anti-ERCC8 antibody (AB275596)
  • WB

Unknown

Western blot - Anti-ERCC8 antibody (AB275596)

Developed using the Odyssey technique.

Performed under reducing conditions.

We are unsure as to the identity of these extra bands.

All lanes:

Western blot - Anti-ERCC8 antibody (ab275596) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Lane 2:

Mouse spleen tissue lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ERCC8. The exact immunogen used to generate this antibody is proprietary information.

Q13216

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/300 - 1/10000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERCC8 also known as CSA protein plays an essential role in the repair of transcription-coupled nucleotide excision repair (TC-NER) pathways. This protein has a mass of about 44 kDa. Expression of ERCC8 is found in several tissues notably in the skin liver and nervous system. It helps in recognizing and repairing DNA damage that occurs during transcription.
Biological function summary

ERCC8 forms a part of the CSA complex which includes other proteins involved in the TC-NER pathway. The CSA complex targets the repair machinery to the site of transcription-blocking lesions. Identifying these lesions is critical to resuming normal transcription processes. The CSA protein therefore actively contributes to maintaining genomic stability by facilitating the correction of transcription-coupled DNA damage.

Pathways

ERCC8 participates directly in the nucleotide excision repair (NER) pathway specifically in its transcription-coupled repair subprocess. This pathway is integral to correcting helix-distorting DNA lesions that impede transcription. ERCC8 interacts closely with the Cockayne syndrome B (CSB) protein and RNA polymerase II during the repair process. This collaboration is important in modulating the restart of transcription post-repair.

Mutations in ERCC8 lead to Cockayne syndrome (CS) characterized by growth defects neurological dysfunction and photosensitivity. ERCC8 alterations are linked primarily to Cockayne syndrome group A. The protein is also involved in repair mechanisms tied to UV-sensitive syndrome. In both conditions faulty ERCC8 function can disrupt normal DNA repair processes leading to severe clinical manifestations associated with DNA repair failure. The role of CSA alongside CSB mutations further highlights its importance in disease phenotypes and the need for effective DNA repair.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes (PubMed : 12732143, PubMed : 16751180, PubMed : 16964240, PubMed : 32142649, PubMed : 34526721, PubMed : 38316879, PubMed : 38600235, PubMed : 38600236). Following recruitment to lesion-stalled RNA polymerase II (Pol II), the CSA complex mediates ubiquitination of Pol II subunit POLR2A/RPB1 at 'Lys-1268', a critical TC-NER checkpoint, governing RNA Pol II stability and initiating DNA damage excision by TFIIH recruitment (PubMed : 12732143, PubMed : 16751180, PubMed : 16964240, PubMed : 32142649, PubMed : 32355176, PubMed : 34526721, PubMed : 38316879, PubMed : 38600235, PubMed : 38600236). The CSA complex also promotes the ubiquitination and subsequent proteasomal degradation of ERCC6/CSB in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair (PubMed : 16751180). Also plays a role in DNA double-strand breaks (DSSBs) repair by non-homologous end joining (NHEJ) (PubMed : 29545921).
See full target information ERCC8
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Product promise

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