Anti-ERCC8 antibody [EPR9237] - BSA and Azide free

• IP

Lab

Immunoprecipitation - Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (AB240096)

This data was developed using ab137033, the same antibody clone in a different buffer formulation.

Purified ab137033 at 1/80 dilution (2μg) immunoprecipitating ERCC8 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab137033 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137033 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 44 kDa

All lanes:

Immunoprecipitation - Anti-ERCC8 antibody [EPR9237] (<a href='/en-us/products/primary-antibodies/ercc8-antibody-epr9237-ab137033'>ab137033</a>)

Predicted band size: 44 kDa

false

• WB

Unknown

Western blot - Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (AB240096)

This data was developed using ab137033, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : ERCC8 knockout HAP1 cell lysate (20 μg)

Lane 3 : HeLa cell lysate (20 μg)

Lane 4 : Molt-4 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab137033 observed at 44 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab137033 was shown to recognize ERCC8 when ERCC8 knockout samples were used, along with additional cross-reactive bands. Wild-type and ERCC8 knockout samples were subjected to SDS-PAGE. ab137033 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively, and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ERCC8 antibody [EPR9237] (<a href='/en-us/products/primary-antibodies/ercc8-antibody-epr9237-ab137033'>ab137033</a>)

Predicted band size: 44 kDa

false

• WB

Unknown

Western blot - Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (AB240096)

This data was developed using ab137033, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ERCC8 antibody [EPR9237] (<a href='/en-us/products/primary-antibodies/ercc8-antibody-epr9237-ab137033'>ab137033</a>) at 1/1000 dilution

Lane 1:

MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 3:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/5000 dilution

Predicted band size: 44 kDa

false