AB153726

Anti-ERF antibody

Name: Anti-ERF antibody (ab153726) | Abcam
Brand: Abcam Limited
SKU: AB153726
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ERF antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ETS domain-containing transcription factor ERF aa 1-300.

View Alternative Names

ETS domain-containing transcription factor ERF, Ets2 repressor factor, PE-2, ERF

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERF antibody (AB153726)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERF antibody (AB153726)

Immunohistochemical analysis of paraffin-embedded U373 xenograft labeling ERF using ab153726 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ERF antibody (AB153726)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERF antibody (AB153726)

HeLa cells stained for ERF (green) using ab153726 at 1/500 dilution in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERF antibody (AB153726)

Paraffin embedded mouse testis tissue stained for ERF using ab153726 at 1/500 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-ERF antibody (AB153726)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ERF antibody (ab153726) at 1/1000 dilution

All lanes:

A431 cell lysate at 30 µg

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ETS domain-containing transcription factor ERF aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P50548

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERF or Ets2 repressor factor is a transcription factor that regulates gene expression by binding to specific DNA sequences. Sometimes referred to as ERF2 this protein has a molecular mass of around 54 kDa. ERF is widely expressed in various tissues including the liver lungs and brain. It localizes mainly in the nucleus where it interacts with DNA to modulate transcriptional activities influencing cellular responses.

Biological function summary

Ets2 repressor factor plays a role in growth regulation. It is known to negatively control the activity of other ETS transcription factors restricting their ability to activate gene expression linked with cell proliferation and differentiation. ERF is not usually part of a larger complex but can form interactions with other regulatory molecules to fine-tune cellular processes. By influencing these pathways ERF helps maintain balanced cell growth and prevent uncontrolled division.

Pathways

ERF integrates into the MAPK/ERK signaling pathway influencing cell cycle control and differentiation. This pathway also involves other proteins such as ERK2 and c-Fos which are important for transmitting signals from extracellular stimuli to the nucleus. Additionally ERF is connected to the TGF-beta signaling pathway where it aids in modulating responses to growth factors that are important in developmental processes and cellular homeostasis.

ERF has implications in cancer progression and developmental syndromes. Dysregulation of ERF gene expression can contribute to increased cell proliferation and tumor growth linking it to cancer pathways. For instance its interaction with ERK2 in the MAPK/ERK pathway influences oncogenic processes. In developmental syndromes mutations in ERF are associated with craniosynostosis a condition affecting skull development often involving altered signaling pathways related to protein FGFR2 which can result in impaired cell differentiation and growth regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation (By similarity).

See full target information ETS domain-containing transcription factor ERF

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:6118 PubMed37059819

2023

Modulation of tau tubulin kinases (TTBK1 and TTBK2) impacts ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Frances M Bashore,Ariana B Marquez,Apirat Chaikuad,Stefanie Howell,Andrea S Dunn,Alvaro A Beltran,Jeffery L Smith,David H Drewry,Adriana S Beltran,Alison D Axtman

Cell reports 26:1286-1302.e8 PubMed30699355

2019

Integrated Phosphoproteome and Transcriptome Analysis Reveals Chlamydia-Induced Epithelial-to-Mesenchymal Transition in Host Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Piotr K Zadora,Cindrilla Chumduri,Koshi Imami,Hilmar Berger,Yang Mi,Matthias Selbach,Thomas F Meyer,Rajendra Kumar Gurumurthy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERF antibody (AB153726)

Immunocytochemistry/ Immunofluorescence - Anti-ERF antibody (AB153726)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERF antibody (AB153726)

Western blot - Anti-ERF antibody (AB153726)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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