AB153731

Anti-eRF1 antibody

Name: Anti-eRF1 antibody (ab153731) | Abcam
Brand: Abcam Limited
SKU: AB153731

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal ERF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ETF1 aa 350 to C-terminus.

View Alternative Names

ERF1, RF1, SUP45L1, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, eRF1, Protein Cl1, TB3-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF1 antibody (AB153731)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF1 antibody (AB153731)

Immunohistochemical analysis of paraffin embedded SW480 xenograft labeling eRF1 using ab153731 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (AB153731)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (AB153731)

Immunofluorescence analysis of A431 cells fixed in 4% paraformaldehyde at RT for 15 min labelling eRF1 protein at cytoplasm using ab153731 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (AB153731)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling eRF1 protein at cytoplasm using ab153731 at a 1/500 dilution (Green).
phalloidin, a cytoskeleton marker, stained by phalloidin antibody at a 1/200 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF1 antibody (AB153731)

Immunohistochemical analysis of mouse testis tissue stained for eRF1 protein at cytoplasm with ab153731 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min.

Supplier Data

Western blot - Anti-eRF1 antibody (AB153731)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-eRF1 antibody (ab153731) at 1/500 dilution

Lane 1:

PC-12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-eRF1 antibody (AB153731)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-eRF1 antibody (ab153731) at 1/500 dilution

Lane 1:

Neuro2a whole cell extracts at 30 µg

Lane 2:

C8D30 whole cell extracts at 30 µg

Lane 3:

NIH-3T3 whole cell extracts at 30 µg

Lane 4:

Raw264.7 whole cell extracts at 30 µg

Lane 5:

C2C12 whole cell extracts at 30 µg

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human ETF1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62495

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "10mM Citrate buffer Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic release factor 1 (eRF1) also known as ETF1 plays a critical role in translation termination during protein synthesis. eRF1 recognizes stop codons on mRNA and helps facilitate the release of the newly synthesized polypeptide chain from the ribosome. This protein has a molecular weight of approximately 48 kDa. Researchers have detected eRF1 in various tissues across eukaryotic organisms highlighting its extensive expression and importance in maintaining cellular function.

Biological function summary

ERF1 functions as a component of the eRF1/eRF3 complex which is essential for the accurate termination of translation. This interaction with eRF3 enhances GTPase activity driving the release process. By binding to stop codons UAA UAG and UGA eRF1 ensures the fidelity of protein synthesis maintaining cellular protein pools correctly. The complex machinery involving eRF1 therefore sustains cellular homeostasis by ensuring proteins are accurately built according to genetic instructions.

Pathways

ERF1 is an important player in the mRNA surveillance and translation termination pathways. The protein's cooperation with eRF3 and SMG1 enables detection and resolution of premature termination codons contributing to nonsense-mediated mRNA decay. Besides eRF1 interacts with ribosomal proteins forming a bridge in the translation machinery allowing it to perform its termination role effectively.

Mutations or dysregulation of eRF1 have potential connections to cancer and neurodegenerative diseases. Abnormal eRF1 levels may lead to faulty translation termination causing the synthesis of aberrant proteins which can contribute to cancer progression. Additionally changes in eRF1 function or expression may have implications in Huntington's disease where protein synthesis disruptions play a role in disease pathogenesis. These associations highlight eRF1's relevance in both cancer biology and neurodegenerative disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons (PubMed : 10676813, PubMed : 16777602, PubMed : 24486019, PubMed : 26245381, PubMed : 27863242, PubMed : 36638793, PubMed : 7990965). The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed : 26245381, PubMed : 27863242, PubMed : 36638793). ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA (PubMed : 26245381, PubMed : 27863242, PubMed : 36638793). Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA (PubMed : 10676813, PubMed : 16777602, PubMed : 26245381, PubMed : 27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed : 19417104). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed : 30682371).

See full target information ETF1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1289175 PubMed38332912

2024

Autoantibodies against eukaryotic translation elongation factor 1 delta in two patients with autoimmune cerebellar ataxia.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Guo,Haitao Ren,Siyuan Fan,Xingchen Chao,Mange Liu,Hongzhi Guan,Jing Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eRF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF1 antibody (AB153731)

Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (AB153731)

Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (AB153731)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF1 antibody (AB153731)

Western blot - Anti-eRF1 antibody (AB153731)

Western blot - Anti-eRF1 antibody (AB153731)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise