Rabbit Polyclonal ERF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ETF1 aa 350 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ETF1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P62495
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes 10mM Citrate buffer Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons (PubMed:10676813, PubMed:16777602, PubMed:24486019, PubMed:26245381, PubMed:27863242, PubMed:36638793, PubMed:7990965). The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed:26245381, PubMed:27863242, PubMed:36638793). ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA (PubMed:26245381, PubMed:27863242, PubMed:36638793). Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA (PubMed:10676813, PubMed:16777602, PubMed:26245381, PubMed:27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:19417104). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371).
Alternative names
ERF1, RF1, SUP45L1, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, eRF1, Protein Cl1, TB3-1
Recommended products
Rabbit Polyclonal ERF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ETF1 aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ETF1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P62495
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The eukaryotic release factor 1 (eRF1) also known as ETF1 plays a critical role in translation termination during protein synthesis. eRF1 recognizes stop codons on mRNA and helps facilitate the release of the newly synthesized polypeptide chain from the ribosome. This protein has a molecular weight of approximately 48 kDa. Researchers have detected eRF1 in various tissues across eukaryotic organisms highlighting its extensive expression and importance in maintaining cellular function.
- Biological function summary
ERF1 functions as a component of the eRF1/eRF3 complex which is essential for the accurate termination of translation. This interaction with eRF3 enhances GTPase activity driving the release process. By binding to stop codons UAA UAG and UGA eRF1 ensures the fidelity of protein synthesis maintaining cellular protein pools correctly. The complex machinery involving eRF1 therefore sustains cellular homeostasis by ensuring proteins are accurately built according to genetic instructions.
- Pathways
ERF1 is an important player in the mRNA surveillance and translation termination pathways. The protein's cooperation with eRF3 and SMG1 enables detection and resolution of premature termination codons contributing to nonsense-mediated mRNA decay. Besides eRF1 interacts with ribosomal proteins forming a bridge in the translation machinery allowing it to perform its termination role effectively.
- Associated diseases and disorders
Mutations or dysregulation of eRF1 have potential connections to cancer and neurodegenerative diseases. Abnormal eRF1 levels may lead to faulty translation termination causing the synthesis of aberrant proteins which can contribute to cancer progression. Additionally changes in eRF1 function or expression may have implications in Huntington's disease where protein synthesis disruptions play a role in disease pathogenesis. These associations highlight eRF1's relevance in both cancer biology and neurodegenerative disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-eRF1 antibody (ab153731)
10% SDS PAGE
All lanes: Western blot - Anti-eRF1 antibody (ab153731) at 1/1000 dilution
All lanes: MOLT4 whole cell lysate at 30 µg
Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF1 antibody (ab153731)
Immunohistochemical analysis of paraffin embedded SW480 xenograft labeling eRF1 using ab153731 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (ab153731)
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling eRF1 using ab153731 at 1/200 dilution.
The image in the right panel was merged with DNA probe.
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