AB31799

Anti-eRF1 antibody

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(4 Publications)

Rabbit Polyclonal ERF1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 4 publications.

View Alternative Names

ERF1, RF1, SUP45L1, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, eRF1, Protein Cl1, TB3-1

3 Images

Project1992****

Western blot - Anti-eRF1 antibody (AB31799)

All lanes:

Western blot - Anti-eRF1 antibody (ab31799) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - Jurkat whole cell lysate (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 20 µg

Lane 3:

Western blot - A-431 whole cell lysate (<a href='/en-us/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Project1992****

Western blot - Anti-eRF1 antibody (AB31799)

All lanes:

Western blot - Anti-eRF1 antibody (ab31799) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 4:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 5:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 6:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

Liver (Rat) Tissue Lysate at 10 µg

Lane 8:

Kidney (Rat) Whole Cell Lysate - normal tissue (<a href='/en-us/products/unavailable/kidney-rat-whole-cell-lysate-normal-tissue-ab29480'>ab29480</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (AB31799)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (AB31799)

ICC/IF image of ab31799 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31799, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic release factor 1 (eRF1) also known as ETF1 plays a critical role in translation termination during protein synthesis. eRF1 recognizes stop codons on mRNA and helps facilitate the release of the newly synthesized polypeptide chain from the ribosome. This protein has a molecular weight of approximately 48 kDa. Researchers have detected eRF1 in various tissues across eukaryotic organisms highlighting its extensive expression and importance in maintaining cellular function.

Biological function summary

ERF1 functions as a component of the eRF1/eRF3 complex which is essential for the accurate termination of translation. This interaction with eRF3 enhances GTPase activity driving the release process. By binding to stop codons UAA UAG and UGA eRF1 ensures the fidelity of protein synthesis maintaining cellular protein pools correctly. The complex machinery involving eRF1 therefore sustains cellular homeostasis by ensuring proteins are accurately built according to genetic instructions.

Pathways

ERF1 is an important player in the mRNA surveillance and translation termination pathways. The protein's cooperation with eRF3 and SMG1 enables detection and resolution of premature termination codons contributing to nonsense-mediated mRNA decay. Besides eRF1 interacts with ribosomal proteins forming a bridge in the translation machinery allowing it to perform its termination role effectively.

Mutations or dysregulation of eRF1 have potential connections to cancer and neurodegenerative diseases. Abnormal eRF1 levels may lead to faulty translation termination causing the synthesis of aberrant proteins which can contribute to cancer progression. Additionally changes in eRF1 function or expression may have implications in Huntington's disease where protein synthesis disruptions play a role in disease pathogenesis. These associations highlight eRF1's relevance in both cancer biology and neurodegenerative disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons (PubMed : 10676813, PubMed : 16777602, PubMed : 24486019, PubMed : 26245381, PubMed : 27863242, PubMed : 36638793, PubMed : 7990965). The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed : 26245381, PubMed : 27863242, PubMed : 36638793). ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA (PubMed : 26245381, PubMed : 27863242, PubMed : 36638793). Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA (PubMed : 10676813, PubMed : 16777602, PubMed : 26245381, PubMed : 27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed : 19417104). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed : 30682371).

See full target information ETF1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2414187121 PubMed39503847

2024

Cytosolic dependent translation supports mitochondrial RNA processing.

Applications

Unspecified application

Species

Unspecified reactive species

Mads M Foged,Emeline Recazens,Sylvain Chollet,Miriam Lisci,George E Allen,Boris Zinshteyn,Doha Boutguetait,Christian Münch,Vamsi K Mootha,Alexis A Jourdain

Nucleic acid therapeutics 29:175-186 PubMed31070517

2019

Targeting Translation Termination Machinery with Antisense Oligonucleotides for Diseases Caused by Nonsense Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Huang,Mariam Aghajan,Tianna Quesenberry,Audrey Low,Susan F Murray,Brett P Monia,Shuling Guo

Cell 176:625-635.e14 PubMed30682371

2019

Regulation of HIV-1 Gag-Pol Expression by Shiftless, an Inhibitor of Programmed -1 Ribosomal Frameshifting.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlu Wang,Yifang Xuan,Yuling Han,Xiang Ding,Kai Ye,Fuquan Yang,Pu Gao,Stephen P Goff,Guangxia Gao

Molecular and cellular biology 30:4245-53 PubMed20606008

2010

Deficiency in a glutamine-specific methyltransferase for release factor causes mouse embryonic lethality.

Applications

WB, IP

Species

Human, Human

Peng Liu,Song Nie,Bing Li,Zhong-Qiang Yang,Zhi-Mei Xu,Jian Fei,Chyuansheng Lin,Rong Zeng,Guo-Liang Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eRF1 antibody

Western blot - Anti-eRF1 antibody (AB31799)

Western blot - Anti-eRF1 antibody (AB31799)

Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (AB31799)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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